Department of TCMs Pharmaceuticals, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
Genes (Basel). 2022 Mar 16;13(3):521. doi: 10.3390/genes13030521.
Diels (), a Chinese perennial plant, is well-known for its medicinal values such as rheumatism, contusion, and anemia. It is rich in bioactive anthraquinones, but the biosynthetic pathways of anthraquinones in remain unknown. To investigate genes involved in anthraquinone biosynthesis in , we generated a de novo transcriptome of using the Illumina HiSeq 2500 sequencing platform. A total of 636,198 transcripts were obtained, in which 140,078 transcripts were successfully annotated. A differential gene expression analysis identified 15 putative genes involved in anthraquinone biosynthesis. Additionally, the hairy roots of were treated with 200 µM Methyl Jasmonate (MeJA). The contents of six bioactive anthraquinones and gene expression levels of 15 putative genes were measured using ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) and real-time quantitative polymerase chain reaction (RT-qPCR), respectively. The results showed that the expressions levels for 11 of the 15 genes and the contents of two of six anthraquinones significantly increased by MeJA treatment. Pearson's correlation analyses indicated that the expressions of 4 of the 15 putative genes were positively correlated with the contents of rubiquinone (Q3) and rubiquinone-3---d-xylopranosyl-(1→6)--d-glucopyranoside (Q20). This study reported the first de novo transcriptome of and shed light on the anthraquinone biosynthesis and genetic information for .
重楼（），一种中国多年生植物，以其治疗风湿、跌打损伤和贫血等功效而闻名。它富含生物活性蒽醌类化合物，但重楼中蒽醌类化合物的生物合成途径尚不清楚。为了研究重楼中参与蒽醌生物合成的基因，我们使用 Illumina HiSeq 2500 测序平台生成了重楼的从头转录组。共获得 636,198 条转录本，其中 140,078 条转录本成功注释。差异基因表达分析鉴定了 15 个可能参与蒽醌生物合成的基因。此外，用 200µM 茉莉酸甲酯（MeJA）处理重楼的毛状根。使用超高效液相色谱-串联质谱（UPLC-MS/MS）和实时定量聚合酶链反应（RT-qPCR）分别测量了六种生物活性蒽醌的含量和 15 个假定基因的表达水平。结果表明，15 个假定基因中有 11 个的表达水平和 6 个蒽醌中的 2 个的含量在 MeJA 处理后显著增加。Pearson 相关分析表明，15 个假定基因中有 4 个的表达与泛醌（Q3）和泛醌-3---二木糖基-（1→6）--D-吡喃葡萄糖苷（Q20）的含量呈正相关。本研究首次报道了重楼的从头转录组，为重楼的蒽醌生物合成和遗传信息提供了线索。
