Department of Obstetrics and Gynecology, Chiayi Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Chiayi 613, Taiwan.
Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, Taiwan.
Medicina (Kaunas). 2022 Mar 4;58(3):386. doi: 10.3390/medicina58030386.
Anti-adhesion barriers are currently used during ovarian cancer surgery to decrease adhesion-related morbidity. Adept (4% icodextrin) solution, a liquid anti-adhesion material, has been widely used during gynecologic surgeries, though the risk of this barrier for oncologic surgery is controversial. The aim of this study was to determine the effect of Adept solution on the proliferation of ovarian cancer cells. We assessed the dose- and time-dependent effects of icodextrin on the growth and proliferation of OVCAR-3 and A2780 human ovarian tumor cell lines in vitro. Cell growth was determined by cell number counting. Expressions of cell cycle-regulation proteins (cyclin D1 and cyclin B1) were determined using Western blot analysis. Adept did not significantly increase ovarian cancer cell growth when tested at various concentrations (0, 1, 5, 10, 15, and 20%, equal to 0, 0.04, 0.2, 0.4, 0.6 and 0.8% icodextrin) and different time points (1-3 days) compared to control cells. Moreover, the protein levels of cyclin D1 and B1 were not overexpression-elevated in icodextrin-treated ovarian cancer cells, either with an increasing concentration or with an increasing treated time. These results demonstrated that Adept does not activate the growth or proliferation of ovarian cancer cells in either a dose- or time-dependent manner. This study supports the use of Adept solution as a safe anti-adhesion barrier for ovarian cancer surgery, though further in vivo studies are necessary.
抗粘连屏障目前用于卵巢癌手术中，以降低与粘连相关的发病率。Adept（4% 异麦芽糖）溶液是一种液体抗粘连材料，已广泛用于妇科手术中，但这种屏障在肿瘤手术中的风险存在争议。本研究旨在确定 Adept 溶液对卵巢癌细胞增殖的影响。我们评估了异麦芽糖对 OVCAR-3 和 A2780 人卵巢肿瘤细胞系体外生长和增殖的剂量和时间依赖性影响。通过细胞计数来确定细胞生长。使用 Western blot 分析来确定细胞周期调节蛋白（cyclin D1 和 cyclin B1）的表达。在不同浓度（0、1、5、10、15 和 20%，相当于 0、0.04、0.2、0.4、0.6 和 0.8%异麦芽糖）和不同时间点（1-3 天）下，Adept 对卵巢癌细胞生长的影响与对照细胞相比均无显著差异。此外，异麦芽糖处理的卵巢癌细胞中 cyclin D1 和 B1 的蛋白水平也没有过表达升高，无论是浓度增加还是处理时间增加。这些结果表明，Adept 既不会以剂量依赖的方式也不会以时间依赖的方式激活卵巢癌细胞的生长或增殖。这项研究支持将 Adept 溶液用作卵巢癌手术中安全的抗粘连屏障，尽管还需要进一步的体内研究。
