中国梨树苹果韧皮部坏死病毒 2 种和柑橘病毒 A 的分子特征及发生率。

Molecular Characteristics and Incidence of Apple Rubbery Wood Virus 2 and Citrus Virus A Infecting Pear Trees in China.

机构信息

Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China.

出版信息

Viruses. 2022 Mar 11;14(3):576. doi: 10.3390/v14030576.

DOI:10.3390/v14030576

PMID:35336983

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8952854/

Abstract

Apple rubbery wood virus 2 (ARWV-2) and citrus virus A (CiVA) belong to a recently approved family in the order and possess negative-sense single-stranded RNA genomes. In this study, the genome sequence of three ARWV-2 isolates (S17E2, LYC2, and LYXS) and a CiVA isolate (CiVA-P) infecting pear trees grown in China were characterized using high-throughput sequencing combined with conventional reverse-transcription PCR (RT-PCR) assays. The genome-wide nt sequence identities were above 93.6% among the ARWV-2 isolates and above 93% among CiVA isolates. Sequence comparisons showed that sequence diversity occurred in the 5' untranslated region of the ARWV-2 genome and the intergenic region of the CiVA genome. For the first time, this study revealed that ARWV-2 proteins Ma and Mb displayed a plasmodesma subcellular localization, and the MP of CiVA locates in cell periphery and can interact with the viral NP in bimolecular fluorescence complementation assays. RT-PCR tests disclosed that ARWV-2 widely occurs, while CiVA has a low incidence in pear trees grown in China. This study presents the first complete genome sequences and incidences of ARWV-2 and CiVA from pear trees and the obtained results extend our knowledge of the viral pathogens of pear grown in China.

摘要

苹果韧皮部泻肿病毒 2 型（ARWV-2）和柑橘病毒 A（CiVA）属于最近批准的目科，拥有负义单链 RNA 基因组。本研究通过高通量测序结合常规逆转录 PCR（RT-PCR）试验，对中国梨树上感染的三种 ARWV-2 分离株（S17E2、LYC2 和 LYXS）和一种 CiVA 分离株（CiVA-P）的基因组序列进行了特征描述。ARWV-2 分离株之间的全基因组 nt 序列同一性在 93.6%以上，而 CiVA 分离株之间的同一性在 93%以上。序列比较表明，ARWV-2 基因组的 5'非翻译区和 CiVA 基因组的基因间隔区发生了序列多样性。本研究首次揭示了 ARWV-2 蛋白 Ma 和 Mb 呈现出质膜小泡的亚细胞定位，而 CiVA 的 MP 位于细胞外周，并能在双分子荧光互补实验中与病毒的 NP 相互作用。RT-PCR 试验揭示了 ARWV-2 广泛存在，而 CiVA 在我国梨树中的发病率较低。本研究首次提供了来自梨树的 ARWV-2 和 CiVA 的完整基因组序列和发病率，研究结果扩展了我们对中国梨树病毒病原体的认识。