Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USA.
Department of Entomology, University of Georgia, Tifton, GA 31793, USA.
Viruses. 2022 Mar 13;14(3):593. doi: 10.3390/v14030593.
Cucurbit chlorotic yellows virus (CCYV) belongs to the genus and is part of a complex of whitefly-transmitted viruses that cause yellowing disease in cucurbits. In the southeastern USA, heavy incidences of CCYV have been observed on all cucurbits grown in the fall. CCYV was detected from wild radish ( L.), a common weed that grows in the southeastern USA by high-throughput sequencing as well as RT-PCR. CCYV sequence from wild radish was 99.90% and 99.95%, identical to RNA 1 and RNA 2 of cucurbit isolates of CCYV from the region. Transmission assays using whiteflies demonstrated that wild radish is a good host for CCYV. Whiteflies were also able to acquire CCYV from wild radish and transmit the virus to cucurbit hosts, which developed typical symptoms associated with CCYV. Using quantitative PCR, the titer of CCYV in wild radish was also estimated to be on par with that of cucurbit hosts of the virus. Whitefly bioassays revealed that wild radish is an acceptable feeding and reproductive host plant. These results indicate that wild radish could serve as a reservoir host for CCYV in the USA and other parts of the world where similar conditions exist.
葫芦科褪绿黄化病毒（CCYV）属于 属，是一组由粉虱传播的病毒的一部分，这些病毒会导致葫芦科植物出现黄化病。在美国东南部，所有秋季种植的葫芦科植物上都观察到了 CCYV 的严重发病率。通过高通量测序和 RT-PCR 检测到，野生萝卜（ L.）中存在 CCYV。野生萝卜的 CCYV 序列与该地区的 CCYV 葫芦科分离物的 RNA1 和 RNA2 完全相同，同源性均为 99.90%和 99.95%。使用粉虱进行的传播测定表明，野生萝卜是 CCYV 的良好宿主。粉虱还能够从野生萝卜中获得 CCYV，并将病毒传播到葫芦科寄主上，这些寄主会出现与 CCYV 相关的典型症状。使用定量 PCR 估计，野生萝卜中 CCYV 的滴度与该病毒的葫芦科寄主相当。粉虱生物测定表明，野生萝卜是一种可接受的取食和繁殖寄主植物。这些结果表明，野生萝卜在美国和其他存在类似条件的国家/地区可能成为 CCYV 的储存宿主。
