Mariani A P, Caserta M T
J Neurocytol. 1986 Oct;15(5):645-55. doi: 10.1007/BF01611863.
With indirect immunofluorescence, glutamate decarboxylase (GAD), the GABA synthesizing enzyme, was localized to cell bodies in the inner half of the inner nuclear layer and a few in the outer tier of the ganglion cell layer in the rhesus monkey retina. In the inner plexiform layer there were three strongly GAD-immunoreactive laminae separated by two less immunoreactive laminae. Electron microscopy demonstrated that the GAD was contained in amacrine cells and these GAD-immunoreactive amacrines were primarily pre- and postsynaptic to biopolar cell axon terminals. The GAD-containing processes possessed small synaptic vesicles and formed synapses that could be characterized as symmetrical. Large, dense-cored vesicles were often found in the cell bodies and synaptic processes of the GAD-immunoreactive amacrine cells. As the vast majority of the synaptic input and output of the GAD-containing amacrine cells was to and from bipolar cells and the strongest GAD-immunoreactivity correlated with the endings of bipolar cells that connect with a single cone, the functional effects of GABA in the primate retina are likely to be found in the responses of single cone pathways in the inner plexiform layer.
采用间接免疫荧光法，在恒河猴视网膜中，γ-氨基丁酸（GABA）合成酶谷氨酸脱羧酶（GAD）定位于内核层内半部分的细胞体以及神经节细胞层外层的少数细胞体。在内网状层中有三个强GAD免疫反应性层，被两个免疫反应性较弱的层隔开。电子显微镜显示，GAD存在于无长突细胞中，这些GAD免疫反应性无长突细胞主要位于双极细胞轴突终末的突触前和突触后。含GAD的突起含有小突触小泡，并形成可被描述为对称性的突触。在GAD免疫反应性无长突细胞的细胞体和突触突起中经常发现大的、有致密核心的小泡。由于绝大多数含GAD的无长突细胞的突触输入和输出是与双极细胞之间进行的，并且最强的GAD免疫反应性与连接单个视锥细胞的双极细胞的终末相关，因此GABA在灵长类视网膜中的功能作用可能体现在内网状层中单个视锥细胞通路的反应中。
