Xu Xiaoxue, Cao Yaqin, Qi Huiying, Deng Daogui, Zhang Ya-Nan, Wu Jianxun, Peng Shuixiu, Zhou Zhongze
School of Life Science Huaibei Normal University Huaibei Anhui China.
School of Resources and Environmental Engineering Anhui University Hefei Anhui China.
Ecol Evol. 2022 Mar 6;12(3):e8685. doi: 10.1002/ece3.8685. eCollection 2022 Mar.
Lake eutrophication and cyanobacterial blooms have become worldwide environmental issues. Under cyanobacterial blooms (especially ), spp. can transfer beneficial information to their offspring in order to improve adaptability. are important regulatory factors of transcription in metazoans, and are involved in the growth and development of organisms. However, the mechanisms of on the expression of in are unclear. In this study, the effects of on expression in the mothers and offspring (F1) of two clones were investigated using a mixed diet of . and . Compared with the 100%S food treatment, the survival rates at the end of the experiment of clone 1-F1 in the food treatments containing . were significantly lower, but it was significantly higher for clone 2-F1 in the 20%M + 80%S food treatment. Moreover, the survival rates at the end of the experiment of clone 1-F1 in the food treatments containing . were significantly higher than those of their mother. Based on previous transcriptome data, 14 of . were identified, including -, -, , -, -, , , , -, , , , , and . The expressions of -, -, , , and of clone 2-mothers in the 40%M + 60%S food treatment were 2.9-22.5 times as high as in the 100%S food treatment, whereas the expressions of -, -, , and of clone 1-mothers were 4.8-13.1 times at same food level. The expression of , , , and of clone 1-F1 in the 40%M + 60%S food treatment was 8.2-21.1 times as high as in the 100%S food treatment. However, compared with the 100%S food treatment, the expressions of - in the mothers and F1 of clone 2 and in the mothers of clone 1 in the food treatments containing . were significantly lower (< .05). Our results suggest that the offspring (F1) produced by . mother pre-exposed to toxic . had stronger adaptability to . than their mothers. Moreover, expressions of . had obvious differences between clones under stress of toxic . .
湖泊富营养化和蓝藻水华已成为全球性环境问题。在蓝藻水华（尤其是）条件下，[具体物种]能够将有益信息传递给后代以提高适应性。[某些物质]是后生动物转录的重要调控因子，参与生物体的生长发育。然而，[某些物质]对[具体物种]中[相关基因]表达的作用机制尚不清楚。在本研究中，使用[具体物质1]和[具体物质2]的混合饲料，研究了[某些物质]对两个[具体物种]克隆的母体及其后代（F1）中[相关基因]表达的影响。与100%S饲料处理相比，含有[具体物质1]的饲料处理中克隆1 - F1在实验结束时的存活率显著降低，但在20%M + 80%S饲料处理中克隆2 - F1的存活率显著更高。此外，含有[具体物质1]的饲料处理中克隆1 - F1在实验结束时的存活率显著高于其母体。基于先前的转录组数据，鉴定出了14个[具体物质1]的[相关基因]，包括 - 、 - 、[多个基因名称]等。在40%M + 60%S饲料处理中，克隆2 - 母体中 - 、 - 、[多个基因名称]的表达是100%S饲料处理中的2.9 - 22.5倍，而在相同饲料水平下，克隆1 - 母体中 - 、 - 、[多个基因名称]的表达是4.8 - 13.1倍。在40%M + 60%S饲料处理中，克隆1 - F1中[多个基因名称]的表达是100%S饲料处理中的8.2 - 21.1倍。然而，与100%S饲料处理相比，在含有[具体物质1]的饲料处理中，克隆2的母体和F1中 - 的表达以及克隆1的母体中[相关基因]的表达显著降低（< 0.05）。我们的结果表明，预先暴露于有毒[具体物质1]的[具体物种]母体所产生的后代（F1）对[具体物质1]的适应性比其母体更强。此外，在有毒[具体物质1]胁迫下，[具体物种]克隆之间[具体物质1]的[相关基因]表达存在明显差异。
