Department of Clinical Laboratory, Shizuoka Children's Hospital, Shizuoka, Japan.
Department of Nephrology, Shizuoka Children's Hospital, Shizuoka, Japan.
Acta Cytol. 2022;66(5):434-440. doi: 10.1159/000521675. Epub 2022 Mar 29.
This study investigated whether our urinary podocyte detection method using podocalyxin (PDX) and Wilms tumor 1 (WT1) immunoenzyme staining combined with liquid-based cytology can serve as a noninvasive routine laboratory test for glomerular disease.
The presence of PDX- and WT1-positive cells was investigated in 79 patients with glomerular disease and 51 patients with nonglomerular disease.
The frequencies and numbers of PDX- and WT1-positive cells were significantly higher in the glomerular disease group than in the nonglomerular disease group. The best cutoffs for PDX- and WT1-positive cell counts for identifying patients with glomerular disease were 3.5 (sensitivity = 67.1% and specificity = 100%) and 1.2 cells/10 mL (sensitivity = 43.0% and specificity = 100%), respectively.
Because our urinary podocyte detection method using PDX immunoenzyme staining can be standardized and it detected glomerular disease with high accuracy, it can likely serve as a noninvasive routine laboratory test for various glomerular diseases.
本研究旨在探讨我们使用足细胞标志蛋白（PDX）和肾母细胞瘤 1 基因（WT1）免疫酶染色联合液基细胞学检测尿足细胞的方法是否可作为一种非侵入性的常规实验室肾小球疾病检测方法。
对 79 例肾小球疾病患者和 51 例非肾小球疾病患者的 PDX 和 WT1 阳性细胞进行了研究。
肾小球疾病组 PDX 和 WT1 阳性细胞的频率和数量明显高于非肾小球疾病组。PDX 和 WT1 阳性细胞计数最佳截断值分别为 3.5（敏感性为 67.1%，特异性为 100%）和 1.2 个/10 mL（敏感性为 43.0%，特异性为 100%），用于诊断肾小球疾病。
由于我们使用 PDX 免疫酶染色检测尿足细胞的方法可以标准化，并且对肾小球疾病具有较高的准确性，因此它可能成为各种肾小球疾病的非侵入性常规实验室检测方法。
