Smirnova E A, Grebenshchikova V I, Chentsov Iu S
Tsitologiia. 1986 Aug;28(8):848-53.
A high tolerance of the cultured SPEV-cells was found under vital examination of their behaviour in hypotonic conditions. The dilution of cultured medium 1:1 hardly affects the cell morphology. With a 1:3 dilution, some minute alterations in the cells were noticed associated with the cell shape and the structure of the nucleus and of the cytoplasm, which later gradually disappear. A marked tendency of morphological alterations in the cells increases with increasing the medium dilution (1:7 and 1:15), however no cell destruction was observed. The complete lysis of the cells occurs only in the medium diluted by 32 times (1:31).
在对培养的SPEV细胞在低渗条件下的行为进行活体检查时,发现其具有较高的耐受性。将培养基1:1稀释几乎不影响细胞形态。当稀释至1:3时,可观察到细胞出现一些与细胞形状、细胞核和细胞质结构相关的细微变化,这些变化随后会逐渐消失。随着培养基稀释度增加(1:7和1:15),细胞形态改变的明显趋势增加,但未观察到细胞破坏现象。细胞完全裂解仅发生在稀释32倍(1:31)的培养基中。