从牛肉生产和加工系统中分离的携带 colibactin 基因（clb）的大肠杆菌的特性。

Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems.

机构信息

Meat Safety and Quality Research Unit, US Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, State Spur 18D, P.O. Box 166, Clay Center, Nebraska, 68933, USA.

出版信息

Sci Rep. 2022 Mar 29;12(1):5305. doi: 10.1038/s41598-022-09274-x.

DOI:10.1038/s41598-022-09274-x

PMID:35351927

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8964808/

Abstract

Certain strains of Escherichia coli possess and express the toxin colibactin (Clb) which induces host mutations identical to the signature mutations of colorectal cancer (CRC) that lead to tumorigenic lesions. Since cattle are a known reservoir of several Enterobacteriaceae including E. coli, this study screened for clb amongst E. coli isolated from colons of cattle-at-harvest (entering beef processing facility; n = 1430), across the beef processing continuum (feedlot to finished subprimal beef; n = 232), and in ground beef (n = 1074). Results demonstrated that clb E. coli were present in cattle and beef. Prevalence of clb E. coli from colonic contents of cattle and ground beef was 18.3% and 5.5%, respectively. clb E. coli were found susceptible to commonly used meat processing interventions. Whole genome sequencing of 54 bovine and beef clb isolates showed clb occurred in diverse genetic backgrounds, most frequently in phylogroup B1 (70.4%), MLST 1079 (42.6%), and serogroup O49 (40.7%).

摘要

某些大肠杆菌菌株拥有并表达毒素 colibactin（Clb），它会诱导宿主发生与结直肠癌（CRC）相同的特征性突变，从而导致肿瘤病变。由于牛是包括大肠杆菌在内的几种肠杆菌科的已知宿主，因此本研究在从屠宰牛的结肠中分离的大肠杆菌（进入牛肉加工设施；n=1430）、牛肉加工过程中（从饲养场到成品副肉；n=232）以及绞碎牛肉（n=1074）中筛选 Clb。结果表明，大肠杆菌 clb 存在于牛和牛肉中。从牛结肠内容物和绞碎牛肉中 clb 大肠杆菌的流行率分别为 18.3%和 5.5%。clb 大肠杆菌对常用的肉类加工干预措施敏感。对 54 株牛和牛肉 clb 分离株的全基因组测序显示，clb 发生在多种遗传背景中，最常见于 phylogroup B1（70.4%）、MLST 1079（42.6%）和 serogroup O49（40.7%）。