[Effects of injected through tail vein on the expressions of biomarkers in neural stem cells and neurons of wild-type rats hippocampus].

To study the effects of (Pg) injected through tail vein on the molecular expression levels of biomarkers of neural stem cells (NSC) and neurons in the hippocampus of wild-type adult rats, and the effects on hippocampal neurogenesis. Eighteen male Sprague-Dawley (SD) rats were randomly divided into 3 groups based on the table of random numbers (6 in each group). In low-intensity group and high-intensity group, rats were injected intravenously through tail vein with 200 μl Pg ATCC33277 [1.0×10 and 1.0×10 colony forming unit (CFU), respectively] 3 times per week for 8 weeks. In the sham group, 200 μl of phosphate buffer saline (PBS) was given instead. Behavioral tests: the navigation and the exploration tests using Morris water maze (MWM) were applied to evaluate learning and memory ability of rats. Immunohistochemistry was performed to detect cells positively expressing nestin, doublecortin (DCX) and neuronal nuclei (NeuN) in the subgranular zone (SGZ) of rats in each group. Western blotting was used to evaluate the expression levels of nestin, DCX and NeuN in rat hippocampus. Learning and memory abilities: on day 5 of navigation test, the lagency time was 22.83 (16.00, 38.34) s in the high-intensity group, significantly longer than the sham group [5.59 (5.41, 6.17) s] (-11.17, 0.001). There were no significant differences between the low-intensity group [9.85 (8.75, 21.01) s] and the sham group (-6.83, 0.080). Results in the exploration test showed that, in the high-intensity group, the number of fime crossing over the previous platform area within 60 s was 1.50 (1.00, 2.00), significantly less than the sham group [4.00 (2.75, 4.00)] (9.75, 0.003); no significant differences between the low-intensity group [2.50 (2.00, 3.00)] and the sham one (4.50, 0.382). Immunohistochemistry showed that the nestin cell density in the low-intensity group [(35.36±4.32) cell/mm] and high-intensity group [(26.51±5.89) cell/mm] were significantly lower than the sham group [(59.58±14.15) cell/mm] (24.21, 0.018; 33.07, 0.005); as for the mean absorbance of DCX cells, the low-intensity group (0.007±0.002) and the high-intensity group (0.006±0.002) were significantly lower than the sham group (0.011±0.001) (0.004, 0.018; 0.006, 0.005); compared with the sham group [(1.13±0.14)×10 cell/mm], the density of NeuN neurons in the high-intensity group [(0.75±0.08)×10 cell/mm] was significantly reduced (0.38, 0.017), and was not significantly changed in the low-intensity group [(0.88±0.19)×10 cell/mm] (0.25, 0.075). Western blotting results showed that, compared with the sham group, the expression levels of nestin, DCX, and NeuN were significantly reduced in the high-intensity group (0.74, 0.001; 0.18, 0.014; 0.35, 0.008), but were not statistically changed in the low-intensity group (0.18, 0.108; 0.08, 0.172; 0.19, 0.077). Pg injected through tail vein may reduce learning and memory abilities of wild-type rats, and may reduce the number of nestin, DCX, and NeuN-positive cells, and the protein expression levels of the above molecules in the hippocampus.