监测环节动物再生过程中端粒的维持。
Monitoring Telomere Maintenance During Regeneration of Annelids.
机构信息
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Department of Life Sciences, National Taiwan University, Taipei, Taiwan.
出版信息
Methods Mol Biol. 2022;2450:467-478. doi: 10.1007/978-1-0716-2172-1_24.
Telomere shortening is a hallmark of aging and eventually constrains the proliferative capacity of cells. The protocols discussed here are used for monitoring telomeres comprehensively in Aeolosoma viride, a model system for regeneration studies. We present methods for analyzing the activity of telomerase enzyme in regenerating tissue by telomeric repeat amplification protocol (TRAP) assay, for comparing telomere length between existing tissue and newly regenerated tissue by telomere restriction fragment (TRF) assay, as well as for visualizing telomeres by fluorescence in situ hybridization (FISH).
端粒缩短是衰老的一个标志，最终会限制细胞的增殖能力。这里讨论的方案用于全面监测 Aeolosoma viride 中的端粒，Aeolosoma viride 是再生研究的模型系统。我们介绍了通过端粒重复扩增协议（TRAP）测定法分析再生组织中端粒酶活性的方法，通过端粒限制片段（TRF）测定法比较现有组织和新再生组织之间的端粒长度的方法，以及通过荧光原位杂交（FISH）可视化端粒的方法。
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