College of Life Sciences, Henan Normal University, Xinxiang, China.
National Centre for Plant Gene Research, State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Beijing, 100101, China.
BMC Plant Biol. 2022 Apr 1;22(1):164. doi: 10.1186/s12870-022-03532-w.
Proliferating cell nuclear antigen (PCNA) is one of the key factors for the DNA replication process and DNA damage repair. Most proteins interacting with PCNA have a common binding motif: PCNA interacting protein box (PIP box). However, some proteins with non-canonical PIP-box have also been reported to be the key factors that interacted with PCNA.
Here we discovered the C terminal of a chromatin-remodeling factor CHR721 with non-canonical PIP-box was essential for interacting with OsPCNA in rice. Both OsPCNA and CHR721 were localized in the nuclei and function in response to DNA damages.
Based on the results and previous work, we proposed a working model that CHR721 with non-canonical PIP-box interacted with OsPCNA and both of them probably participate in the DNA damage repair process.
增殖细胞核抗原（PCNA）是 DNA 复制过程和 DNA 损伤修复的关键因素之一。与 PCNA 相互作用的大多数蛋白质都具有一个常见的结合基序：PCNA 相互作用蛋白盒（PIP 盒）。然而，一些具有非典型 PIP 盒的蛋白质也被报道为与 PCNA 相互作用的关键因素。
在这里，我们发现染色质重塑因子 CHR721 的 C 端具有非典型的 PIP 盒，对于在水稻中与 OsPCNA 相互作用是必不可少的。OsPCNA 和 CHR721 都定位于细胞核中，并在响应 DNA 损伤时发挥作用。
基于这些结果和以前的工作，我们提出了一个工作模型，即具有非典型 PIP 盒的 CHR721 与 OsPCNA 相互作用，它们可能都参与 DNA 损伤修复过程。
