Suter M, Butler J E
Immunol Lett. 1986 Nov 3;13(6):313-6. doi: 10.1016/0165-2478(86)90064-7.
The studies reported here describe a model system by which the effect of surface adsorption on the antigen capture capacity (AgCC) of monoclonal antibodies (MoAbs) can be quantitated. Three mouse anti-fluorescein (FLU) MoAbs were biotinylated and iodinated with [125I]Na. The AgCC/ng of these MoAbs was compared when they were adsorbed directly on plastic or immobilized via a Protein Avidin Biotin Capture (PABC) system. In this system, biotinylated MoAbs were allowed to adsorb on biotinylated carrier proteins through a "bridging" process using Streptavidin. This permits MoAbs to complex with antigen at some distance from the plastic surface. The AgCCs of the MoAbs immobilized using the PABC system were 5-400-fold higher than when they were directly adsorbed on plastic.
此处报道的研究描述了一种模型系统，通过该系统可以定量表面吸附对单克隆抗体（MoAb）抗原捕获能力（AgCC）的影响。三种小鼠抗荧光素（FLU）单克隆抗体用生物素进行标记并用[125I]Na进行碘化。当这些单克隆抗体直接吸附在塑料上或通过蛋白质抗生物素蛋白-生物素捕获（PABC）系统固定时，比较了它们的每纳克AgCC。在该系统中，生物素化的单克隆抗体通过使用链霉抗生物素蛋白的“桥连”过程吸附在生物素化的载体蛋白上。这使得单克隆抗体能够在距塑料表面一定距离处与抗原形成复合物。使用PABC系统固定的单克隆抗体的AgCC比直接吸附在塑料上时高5至400倍。
