Rombout J H, Bol J, Taverne-Thiele J J
Histochemistry. 1986;85(6):467-73. doi: 10.1007/BF00508428.
Using the semi-thin/ultra-thin technique six different immunoreactive endocrine cell types are ultrastructurally identified in 0.5% glutaraldehyde fixed gut of B. conchonius. In addition two of them (gastrin- and PP-immunoreactive cells) are also characterized with the immunogold method, showing that the immunoreactivity is only restricted to the secretory granules. Size distribution histograms and the average diameters of 30% (d30) of the largest granules are given, showing a gradual increase in granule size from unspecific immunoreactive cells, (d30 = 110 nm) via gastrin- (119 nm), VIP-like- (127 nm), met-enkephalin- (143 nm) and PP- (174 nm) to glucagon-immunoreactive cells (178 nm). The presence of PP- and glucagon-immunoreactivity in the same cells and the consequence for their granule size is discussed. In the distal part of the gut endocrine cells are found showing no immunoreactivity with the antisera used; their granules (d30 = 144 nm) were, although not significantly, larger then those of VIP-like-immunoreactive cells, also found in that part of the gut. It is supposed that they represent substance P-immunoreactive cells. Unfortunately, secretory granules of several cell types showed about 20% more shrinkage in 0.5% glutaraldehyde fixed tissue, than in osmicated tissue.
运用半薄/超薄技术，在0.5%戊二醛固定的猪鼻蛙肠道中，超微结构鉴定出六种不同的免疫反应性内分泌细胞类型。此外，其中两种细胞（胃泌素免疫反应性细胞和PP免疫反应性细胞）也通过免疫金法进行了表征，结果表明免疫反应性仅局限于分泌颗粒。给出了30%最大颗粒（d30）的大小分布直方图和平均直径，显示颗粒大小从非特异性免疫反应性细胞（d30 = 110 nm）经胃泌素免疫反应性细胞（119 nm）、类血管活性肠肽免疫反应性细胞（127 nm）、甲硫氨酸脑啡肽免疫反应性细胞（143 nm）和PP免疫反应性细胞（174 nm）到胰高血糖素免疫反应性细胞（178 nm）逐渐增大。讨论了同一细胞中PP和胰高血糖素免疫反应性的存在及其对颗粒大小的影响。在肠道远端发现了一些内分泌细胞，它们与所用抗血清无免疫反应；其颗粒（d30 = 144 nm）虽然差异不显著，但比在肠道该部位发现的类血管活性肠肽免疫反应性细胞的颗粒大。推测它们代表P物质免疫反应性细胞。遗憾的是，几种细胞类型的分泌颗粒在0.5%戊二醛固定组织中的收缩比在锇酸处理组织中多约20%。
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