Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
China Resources Sanjiu Medical & Pharmaceutical Co., Ltd., Shenzhen 518110, China.
Chin J Nat Med. 2022 Mar;20(3):202-209. doi: 10.1016/S1875-5364(21)60098-5.
Two cardenolide glycosides, corotoxigenin 3-O-[β-D-glucopyranosyl-(1→4)-6-deoxy-β-D-glucopyranoside] (1) and coroglaucigenin 3-O-[β-D-glucopyranosyl-(1→4)-6-deoxy-β-D-glucopyranoside] (2), were isolated from the seed fairs of Asclepias curassavica. The structures of 1-2 were determined based on the combination of the analysis of their MS, NMR spectroscopic data and acid hydrolysis. The inhibitory effects of compounds 1 and 2 on human colorectal carcinoma cells (HCT116), non-small cell lung carcinoma cells (A549) and hepatic cancer cells (SMMC-7721) were evaluated. The results showed that both compounds 1 and 2 significantly inhibited the viability, proliferation, and migration of A549, HCT116 and SMMC-7721 cells, suggesting that compounds 1 and 2 can be applied in the treatment of lung, colon and liver cancers in clinical practice. This study may not only provide a scientific basis for clarifying the active ingredients in A. curassavica, but also help to understand its antitumor activity, which can promote the application of A. curassavica in clinical treatment of various cancers.
从马利筋种子中分离得到两个卡烯内酯糖苷，即考托苷元 3-O-[β-D-吡喃葡萄糖基-(1→4)-6-去氧-β-D-吡喃葡萄糖苷]（1）和考格鲁吉宁 3-O-[β-D-吡喃葡萄糖基-(1→4)-6-去氧-β-D-吡喃葡萄糖苷]（2）。根据它们的 MS、NMR 光谱数据和酸水解分析的综合分析，确定了 1-2 的结构。测定了化合物 1 和 2 对人结直肠癌细胞（HCT116）、非小细胞肺癌细胞（A549）和肝癌细胞（SMMC-7721）的抑制作用。结果表明，化合物 1 和 2 均能显著抑制 A549、HCT116 和 SMMC-7721 细胞的活力、增殖和迁移，表明化合物 1 和 2 可应用于临床治疗肺癌、结肠癌和肝癌。本研究不仅可为阐明马利筋中的活性成分提供科学依据，而且有助于了解其抗肿瘤活性，可促进马利筋在治疗各种癌症的临床应用。
