酵母RNA聚合酶B对DNA-组蛋白复合物的转录
Transcription of DNA-histone complexes by yeast RNA polymerase B.
作者信息
Karagyozov L K, Valkanov M A, Hadjiolov A A
出版信息
Nucleic Acids Res. 1978 Jun;5(6):1907-17. doi: 10.1093/nar/5.6.1907.
Abstract
Transcription of denatured DNA complexed with histones (total, H1 or H2A/H2B/H3/H4) by yeast RNA polymerase B is investigated. Binding of histones to DNA restricts its template activity by decreasing the formation of active, heparin-resistant, RNA polymerase initiation complexes. The elongation of pre-initiated RNA on denatured DNA, complexed with histones, is possible, although resulting in somewhat shorter RNA chains. It is suggested that RNA polymerase B can elongate on a DNA strand covered with histones.
摘要
对酵母RNA聚合酶B转录与组蛋白（全部、H1或H2A/H2B/H3/H4）复合的变性DNA进行了研究。组蛋白与DNA的结合通过减少活性、肝素抗性RNA聚合酶起始复合物的形成来限制其模板活性。与组蛋白复合的变性DNA上预起始RNA的延伸是可能的，尽管会产生稍短的RNA链。有人提出RNA聚合酶B可以在覆盖有组蛋白的DNA链上延伸。
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