研究采用新型筛查方法调查 Barrett 食管中人类乳头瘤病毒的流行情况。
Study to investigate the prevalence of human papillomavirus in Barrett's oesophagus using a novel screening methodology.
机构信息
NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and the University of Nottingham, Nottingham, UK
NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and the University of Nottingham, Nottingham, UK.
出版信息
BMJ Open Gastroenterol. 2022 Apr;9(1). doi: 10.1136/bmjgast-2021-000840.
INTRODUCTION
Human papillomavirus (HPV) is strongly associated with Barrett's dysplasia and oesophageal cancer suggesting a role in carcinogenesis. HPV persistence predicts treatment failure after endotherapy for Barrett's dysplasia. This pilot study applies a novel HPV screening tool (previously only used in the oropharynx) to detect HPV DNA directly and determine the prevalence rates in Barrett's oesophagus (BO).
METHOD
DNA was extracted from 20 formalin-fixed BO samples. HPV DNA was detected using real-time PCR and gel electrophoresis.
RESULTS
5 out of 20 patients were identified as positive for HPV. Prevalence was 25% in patients with BO.
CONCLUSION
This method can be used in BO's tissue to determine HPV infection. Adoption of this as a screening test could potentially revolutionise future research in this area. If a clear link between HPV and Barrett's dysplasia can be confirmed, this qPCR method has the potential to aid in monitoring and/or dysplasia detection by stratifying those most at risk and aid in the development of new therapies.
简介
人乳头瘤病毒（HPV）与 Barrett 发育不良和食管癌密切相关，提示其在致癌作用中的作用。HPV 持续存在预测 Barrett 发育不良内镜治疗后的治疗失败。这项初步研究应用了一种新的 HPV 筛查工具（以前仅用于口咽），直接检测 HPV DNA，并确定 Barrett 食管（BO）中的 HPV 流行率。
方法
从 20 例福尔马林固定的 BO 样本中提取 DNA。使用实时 PCR 和凝胶电泳检测 HPV DNA。
结果
20 例患者中有 5 例 HPV 阳性。BO 患者的患病率为 25%。
结论
该方法可用于 BO 组织中确定 HPV 感染。采用这种筛查试验可能会彻底改变该领域未来的研究。如果能够明确 HPV 与 Barrett 发育不良之间的联系，这种 qPCR 方法有可能通过对高危人群进行分层来帮助监测和/或检测发育不良，并有助于开发新的治疗方法。
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