用于检测痢疾志贺氏菌志贺毒素及相关毒素的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins.
作者信息
Kongmuang U, Honda T, Miwatani T
出版信息
J Clin Microbiol. 1987 Jan;25(1):115-8. doi: 10.1128/jcm.25.1.115-118.1987.
Abstract
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detection of Shiga toxin. Four species of Shigella, Escherichia coli, and Vibro parahaemolyticus were tested for production of Shiga or Shiga-like toxin by ELISA and Vero cell bioassay. In the ELISA, most strains of S. dysenteriae and some strains of E. coli isolated from traveler's diarrhea were positive. These ELISA-positive strains were positive by Vero cell bioassay without exception. Some E. coli strains and most V. parahaemolyticus strains were toxic to Vero cells, although they were negative in the ELISA. Much of the cytotoxic activity was not neutralized by anti-Shiga toxin antiserum. The newly developed sandwich ELISA is specific and can be a substitute for the cumbersome Vero cell bioassay.
摘要
开发了一种用于检测志贺毒素的夹心酶联免疫吸附测定（ELISA）。通过ELISA和Vero细胞生物测定法检测了四种志贺氏菌、大肠杆菌和副溶血性弧菌产生志贺毒素或志贺样毒素的情况。在ELISA中，痢疾志贺氏菌的大多数菌株以及从旅行者腹泻中分离出的一些大肠杆菌菌株呈阳性。这些ELISA阳性菌株无一例外在Vero细胞生物测定中也呈阳性。一些大肠杆菌菌株和大多数副溶血性弧菌菌株对Vero细胞有毒性，尽管它们在ELISA中呈阴性。许多细胞毒性活性不能被抗志贺毒素抗血清中和。新开发的夹心ELISA具有特异性，可替代繁琐的Vero细胞生物测定法。
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