CommSensLab, Universitat Politecnica de Catalunya (UPC), 08034 Barcelona, Spain.
Department of Research and Innovation, Fundació Privada Hospital Asil de Granollers, 08402 Granollers, Spain.
Sensors (Basel). 2022 Mar 22;22(7):2441. doi: 10.3390/s22072441.
Detection of bioparticles is of great importance in electrophoresis, identification of biomass sources, food and water safety, and other areas. It requires a proper model to describe bioparticles' electromagnetic characteristics. A numerical study of bacteria during their functional activity was carried out by using two different geometrical models for the cells that considered the bacteria as layered ellipsoids and layered spheres. It was concluded that during cell duplication, the change in the dielectric permittivity of the cell is high enough to be measured at radio frequencies of the order of 50 kHz. An experimental setup based on the capacitive Wheatstone bridge was designed to measure relative changes in permittivity during cell division. In this way, the theoretical model was validated by measuring the dielectric permittivity changes in a cell culture of ATTC 8739 from WDCM 00012 Vitroids. The spheroidal model was confirmed to be more accurate.
在电泳、生物质源识别、食品和水安全等领域,生物粒子的检测非常重要。这需要一个合适的模型来描述生物粒子的电磁特性。本研究使用了两种不同的细胞几何模型,即分层椭球体和分层球体,对细菌在功能活动期间进行了数值研究。结果表明,在细胞复制过程中,细胞介电常数的变化足以在 50 kHz 左右的射频频率下进行测量。设计了一种基于电容惠斯通电桥的实验装置,以测量细胞分裂过程中介电常数的相对变化。通过测量 WDCM 00012 Vitroids 中的 ATTC 8739 细胞培养物的介电常数变化,对理论模型进行了验证。结果证实,球形模型更准确。