Department of Biochemistry, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Liver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
PLoS One. 2022 Apr 12;17(4):e0266227. doi: 10.1371/journal.pone.0266227. eCollection 2022.
Non-alcoholic fatty liver disease (NAFLD) is associated with an increased risk of cardiovascular events. HDL exerts various protective functions on the cardiovascular system including anti-inflammatory activity by suppressing adhesion molecules expression in inflammation-induced endothelial cells. This study was designed to search if the anti-inflammatory capacity of apolipoprotein B-depleted plasma (apoB-depleted plasma) is altered in NAFLD patients.
A total of 83 subjects including 42 NAFLD and 41 control subjects were included in this cross-sectional study. Anti-inflammatory function of HDL was determined as the ability of apoB-depleted plasma to inhibit tumor necrosis factor-α (TNF-α)-induced expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs).
Incubation of inflammation-stimulated HUVECs with the NAFLD patients' apo-B depleted plasma led to higher levels of expression of adhesion molecules compared to the control subjects' plasma samples, reflecting an impaired anti-inflammatory capacity of apoB-depleted plasma in the NAFLD patients. Impaired anti-inflammatory capacity of apoB-depleted plasma was correlated with fatty liver and obesity indices. After adjustment with obesity indices, the association of anti-inflammatory capacity of apoB-depleted plasma with NAFLD remained significant.
Impaired anti-inflammatory activity of apoB-depleted plasma was independently associated with NAFLD.
非酒精性脂肪性肝病（NAFLD）与心血管事件风险增加相关。HDL 在心血管系统中发挥多种保护功能，包括通过抑制炎症诱导的内皮细胞中黏附分子的表达来发挥抗炎活性。本研究旨在探讨 NAFLD 患者载脂蛋白 B 缺乏血浆（apoB-depleted plasma）的抗炎能力是否发生改变。
本横断面研究共纳入 83 例受试者，包括 42 例 NAFLD 患者和 41 例对照组。apoB 缺乏血浆的抗炎功能通过测定其抑制肿瘤坏死因子-α（TNF-α）诱导人脐静脉内皮细胞（HUVECs）黏附分子表达的能力来确定。
与对照组血浆样本相比，炎症刺激的 HUVECs 与 NAFLD 患者的 apo-B 缺乏血浆孵育后，黏附分子的表达水平更高，这反映了 apoB 缺乏血浆在 NAFLD 患者中的抗炎能力受损。apoB 缺乏血浆的抗炎能力受损与脂肪肝和肥胖指数相关。在调整肥胖指数后，apoB 缺乏血浆抗炎能力与 NAFLD 的相关性仍然显著。
apoB 缺乏血浆的抗炎活性受损与 NAFLD 独立相关。
