State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, PR China; South China Sea Fisheries Research Institute (CAFS), Guangzhou, PR China.
Dev Comp Immunol. 2022 Aug;133:104411. doi: 10.1016/j.dci.2022.104411. Epub 2022 Apr 18.
The Wnt family genes are essentially implicated in development and growth in animals. Accumulating clues have pointed to the importance of Wnts in invertebrate immunity, but the underlying mechanisms are still unclear to date. The Wnt5b has been known to promote white spot syndrome virus (WSSV) infection in shrimp but its role in antibacterial response remains unclear. In the current study, we focused on the involvement of Wnt5b in Vibrio parahaemolyticus infection in Pacific white shrimp Penaeus vannamei. We demonstrated that the expression of Wnt5b was regulated by the IMD-Relish and JAK-STAT pathways but not the Dorsal pathway and was suppressed upon bacterial infection. Although Wnt5b did not affect the cellular immunity in shrimp, it was involved in regulation of humoral immunity. Silencing of Wnt5b in vivo significantly increased expression of several antimicrobial peptides but decreased that of many immune functional proteins including C-type lectins and lysozymes. Treatment with recombinant Wnt5b protein increased the susceptibility of shrimp to V. parahaemolyticus infection, while silencing of Wnt5b in vivo showed an opposite result. These suggested that Wnt5b plays a negative role in antibacterial response in shrimp. Together with previous reports, the current study shows that Wnt5b functions as an inhibitor for shrimp immunity, which is a potential target for improving immune responses against infection.
Wnt 家族基因在动物的发育和生长中起着重要作用。越来越多的线索表明 Wnts 在无脊椎动物免疫中具有重要作用，但迄今为止，其潜在机制仍不清楚。已知 Wnt5b 可促进虾类感染白斑综合征病毒（WSSV），但其在抗菌反应中的作用尚不清楚。在本研究中，我们专注于 Wnt5b 在凡纳滨对虾感染副溶血弧菌中的作用。我们证明了 Wnt5b 的表达受 IMD-Relish 和 JAK-STAT 途径调节，但不受 Dorsal 途径调节，并在细菌感染后受到抑制。虽然 Wnt5b 不影响虾的细胞免疫，但它参与了体液免疫的调节。体内沉默 Wnt5b 显著增加了几种抗菌肽的表达，但降低了许多免疫功能蛋白的表达，包括 C 型凝集素和溶菌酶。重组 Wnt5b 蛋白的处理增加了虾对副溶血弧菌感染的易感性，而体内沉默 Wnt5b 则表现出相反的结果。这表明 Wnt5b 在虾的抗菌反应中发挥负作用。结合以前的报道，本研究表明 Wnt5b 作为虾类免疫的抑制剂发挥作用，这是提高抗感染免疫反应的潜在目标。
