Formulation of Artificial Diets for Mass-Rearing Walker (Lepidoptera: Pyralidae) Using the Carcass Milling Technique.

The carcass milling technique (CMT) formulates specific diets from nutrient analyses of species to be reared and their natural host plants. The first of four diets developed used the minimum ingredient specifications (MS) of published diets for Eldana saccharina. The remaining were based on the ideal amino acid composition and profile (IAAP) of its second (IAAP2), third/fourth (IAAP3/4) and fifth/sixth (IAAP5/6) instar larvae. The control was a modified Ostrinia nubilalis diet. Survival to pupae of inoculated E saccharina neonates was high on all CMT formulations (>92% at day 20 and >97% at day 27). Larvae developed fastest on the IAAP3/4 and MS diets (25% and 17% prepupae and pupae on day 20, respectively). Pupal weights were not significantly influenced by CMT diets (0.1121 g male; 0.1864 g female). The control group produced heavier male and female pupae (0.1204 g; 0.2085 g, respectively). Adult emergence was highest from the MS (98%), then the IAAP3/4 (97%) and control (96%) diets. Sex ratio of adults from all diets was close to one. Males from the IAAP5/6 diet mated with significantly more females (six), and females from the MS diet mated with more males (three) than those from remaining formulations. All females produced in excess of 870 eggs, more than 90% were fertile after mating. The pH (4.79); moisture content (81.43%) and water activity (0.92 aw) of the diets were not significantly different, maintaining quality and stability throughout the larval period, ensuring optimal growth and development. The MS diet formulation was the preferred choice to replace the current E. saccharina diet.