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蜜蜂咽下腺中主要蜂王浆蛋白在摄入细菌后的差异表达

Differential Expression of Major Royal Jelly Proteins in the Hypopharyngeal Glands of the Honeybee upon Bacterial Ingestion.

作者信息

Kim Yun-Hui, Kim Bo-Yeon, Kim Jin-Myung, Choi Yong-Soo, Lee Man-Young, Lee Kwang-Sik, Jin Byung-Rae

机构信息

College of Natural Resources and Life Science, Dong-A University, Busan 49315, Korea.

Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.

出版信息

Insects. 2022 Mar 29;13(4):334. doi: 10.3390/insects13040334.

DOI:10.3390/insects13040334
PMID:35447776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9025693/
Abstract

Honeybee vitellogenin (Vg) transports pathogen fragments from the gut to the hypopharyngeal glands and is also used by nurse bees to synthesize royal jelly (RJ), which serves as a vehicle for transferring pathogen fragments to the queen and young larvae. The proteomic profile of RJ from bacterial-challenged and control colonies was compared using mass spectrometry; however, the expression changes of major royal jelly proteins (MRJPs) in hypopharyngeal glands of the honeybee in response to bacterial ingestion is not well-characterized. In this study, we investigated the expression patterns of in the fat body and in the hypopharyngeal glands of nurse bees after feeding them live or heat-killed . The expression levels of and in the hypopharyngeal glands were upregulated along with in the fat body of nurse bees fed with live or heat-killed over 12 h or 24 h. We observed that the expression patterns of and in the hypopharyngeal glands and in the fat body of nurse bees upon bacterial ingestion were differentially expressed depending on the bacterial status and the time since bacterial ingestion. In addition, the genes had increased expression in young larvae fed heat-killed . Thus, our findings indicate that bacterial ingestion upregulates the transcriptional expression of in the hypopharyngeal glands as well as in the fat body of nurse bees.

摘要

蜜蜂卵黄原蛋白（Vg）将病原体片段从肠道运输到下咽腺，并且被哺育蜂用于合成蜂王浆（RJ），蜂王浆作为一种载体将病原体片段传递给蜂王和幼虫。使用质谱法比较了来自受细菌挑战的蜂群和对照蜂群的蜂王浆的蛋白质组概况；然而，蜜蜂下咽腺中主要蜂王浆蛋白（MRJPs）响应细菌摄入后的表达变化尚未得到充分表征。在本研究中，我们调查了给哺育蜂喂食活的或热灭活的[细菌名称未给出]后，其脂肪体和下咽腺中[相关基因未给出]的表达模式。在12小时或24小时内，给哺育蜂喂食活的或热灭活的[细菌名称未给出]后，其下咽腺中[相关基因未给出]和[相关基因未给出]的表达水平与脂肪体中的[相关基因未给出]一起上调。我们观察到，哺育蜂在摄入细菌后，下咽腺中[相关基因未给出]和[相关基因未给出]以及脂肪体中[相关基因未给出]的表达模式根据细菌状态和摄入细菌后的时间而差异表达。此外，[相关基因未给出]基因在喂食热灭活的[细菌名称未给出]的幼虫中表达增加。因此，我们的研究结果表明，摄入细菌会上调哺育蜂下咽腺中[相关基因未给出]以及脂肪体中[相关基因未给出]的转录表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6076/9025693/1cea2eb0efbe/insects-13-00334-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6076/9025693/e071d93aaf42/insects-13-00334-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6076/9025693/f11519a89ca8/insects-13-00334-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6076/9025693/1cea2eb0efbe/insects-13-00334-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6076/9025693/e071d93aaf42/insects-13-00334-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6076/9025693/f11519a89ca8/insects-13-00334-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6076/9025693/1cea2eb0efbe/insects-13-00334-g003.jpg

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