National Engineering Laboratory for Animal Breeding, MOA Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, China.
Genes (Basel). 2022 Apr 10;13(4):668. doi: 10.3390/genes13040668.
Castration can significantly enhance fat deposition in pigs, and the molecular mechanism of fat deposition caused by castration and its influence on fat deposition in different parts of pigs remain unclear. RNA-seq was performed on adipose tissue from different parts of castrated and intact Yorkshire pigs. Different ceRNA networks were constructed for different fat parts. GO and KEGG pathway annotations suggested that testosterone elevates cell migration and affects differentiation and apoptosis in back fat, while it predisposes animals to glycolipid metabolism disorders and increases the expression of inflammatory cytokines in abdominal fat. The interaction between M-7474, novel_miR_243 and was verified by dual fluorescence experiments. This ceRNA relationship has also been demonstrated in porcine preadipocytes. Overexpression of M-7474 significantly inhibited the differentiation of preadipocytes compared to the control group. When 100 nM testosterone was added during preadipocyte differentiation, the expression of M-7474 was increased, and preadipocyte differentiation was significantly inhibited. Testosterone can affect preadipocyte differentiation by upregulating the expression of M-7474, sponging novel-miR-243, and regulating the expression of genes such as SGK1. At the same time, HSD11B1 and SLC2A4 may also be regulated by the corresponding lncRNA and miRNA, which ultimately affects glucose uptake by adipocytes and leads to obesity.
去势可以显著促进猪的脂肪沉积，而去势引起的脂肪沉积的分子机制及其对猪不同部位脂肪沉积的影响尚不清楚。对去势和完整约克夏猪不同部位脂肪组织进行了 RNA-seq 分析。为不同的脂肪部位构建了不同的 ceRNA 网络。GO 和 KEGG 通路注释表明，睾丸酮可促进细胞迁移，并影响背脂的分化和凋亡，而使动物易患糖脂代谢紊乱，并增加腹脂中炎症细胞因子的表达。通过双荧光实验验证了 M-7474、novel_miR_243 和 的相互作用。这种 ceRNA 关系也在猪前体脂肪细胞中得到了证实。与对照组相比，M-7474 的过表达显著抑制了前体脂肪细胞的分化。在前体脂肪细胞分化过程中加入 100 nM 睾丸酮时，M-7474 的表达增加，前体脂肪细胞分化明显受到抑制。睾丸酮可以通过上调 M-7474 的表达、海绵新型 miR-243 并调节 SGK1 等基因的表达来影响前体脂肪细胞的分化。同时，HSD11B1 和 SLC2A4 也可能受到相应的 lncRNA 和 miRNA 的调节，最终影响脂肪细胞的葡萄糖摄取，导致肥胖。
