Wang Wenjing, Niu Longjian, Hou Chunhui
Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.
School of Life Science and Stake Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.
Methods Mol Biol. 2022;2484:55-67. doi: 10.1007/978-1-0716-2253-7_5.
High-throughput chromosome conformation capture (Hi-C) enables the global quantification of chromatin interaction frequency in eukaryotic nuclei. This method is based on in situ Hi-C, in which chromatin is cross-linked with formaldehyde, then digested with restriction enzyme. Biotin-labeled nucleotide is incorporated before the spatially adjacent DNA ends are ligated, making it possible to enrich specifically the chimeric ligation products for deep sequencing. In this chapter, we describe a modified in situ Hi-C protocol for the global chromatin interaction analysis in plants.
高通量染色体构象捕获技术（Hi-C）能够对真核细胞核中的染色质相互作用频率进行全局定量。该方法基于原位Hi-C，即染色质先用甲醛交联，然后用限制性酶消化。在空间上相邻的DNA末端连接之前掺入生物素标记的核苷酸，从而能够特异性富集嵌合连接产物以进行深度测序。在本章中，我们描述了一种经过改进的原位Hi-C方案，用于植物中的全局染色质相互作用分析。
