使用3,3'-二氨基联苯胺和1-萘酚碱性染料在同一组织切片中同时和连续定位两种抗原。
Simultaneous and successive localization of two antigens in the same tissue section using 3,3'-diaminobenzidine and 1-naphthol basic dye.
作者信息
Mauro A, Bulfone A, Giaccone G, Schiffer D
出版信息
Histochemistry. 1986;86(2):123-6. doi: 10.1007/BF00493376.
We describe two procedures for the simultaneous and successive localization of two antigens in the same tissue section. In the simultaneous staining procedure, the first antigen was localized using 3,3'-diaminobenzidine (DAB), while the second antigen was stained using the 1-naphthol basic dye (1-NBD) method. The colour of the second antigen depended on the basic dye used, and no mixing of colours was observed when the two antigens were localized in different cells or structures. However, sequential double staining proved to be more convenient for the demonstration of two antigens in the same cell. In this procedure, the first antigen was stained using 1-NBD, and the interesting microscopic fields were photographed. The basic dye was then completely removed, and the second antigen was stained using DAB.
我们描述了在同一组织切片中同时和连续定位两种抗原的两种方法。在同时染色法中，第一种抗原使用3,3'-二氨基联苯胺（DAB）进行定位，而第二种抗原使用1-萘酚碱性染料（1-NBD）法进行染色。第二种抗原的颜色取决于所使用的碱性染料，当两种抗原定位在不同细胞或结构中时未观察到颜色混合。然而，连续双重染色被证明对于在同一细胞中显示两种抗原更为方便。在该方法中，第一种抗原使用1-NBD进行染色，并对感兴趣的显微镜视野进行拍照。然后将碱性染料完全去除，第二种抗原使用DAB进行染色。
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