Sun Xiaowei, Xue Fenqin, Wen Jialin, Gao Limin, Li Yang, Jiang Qianqian, Yang Lijun, Cui Hong
Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Core Facility Center, Capital Medical University, Beijing, China.
Front Pediatr. 2022 Apr 7;10:837909. doi: 10.3389/fped.2022.837909. eCollection 2022.
Perinatal hypoxic-ischemic encephalopathy (HIE) is a major cause of epilepsy and chronic neurologic morbidity in premature infants. This study aimed to investigate the characteristics of acute seizures and the pattern of background activity on amplitude-integrated electroencephalography (aEEG) in neonatal rats with HIE.
Hypoxia-ischemia (HI) was induced in postnatal day (P) 3 neonatal rats (n = 12) by ligation of the left carotid artery and exposure to airtight hypoxia for 2 h. Data regarding seizure type, frequency, and duration and those related to neurobehavioral development were collected, and the integrated power of background EEG was analyzed to evaluate the effect of HI.
All neonatal rats in the HI group experienced frequent seizures during hypoxia, and 83.3% of rats (10/12) experienced seizures immediately after hypoxia. Seizure frequency and duration gradually decreased with increasing age. The mortality rate of the HI group was 8.33% (1/12); 120 h after HI induction, only 27.3% (3/11) of pups had low-frequency and short-duration electrographic seizures, respectively. HI rats, which presented seizure activities 96 h after HI insult, exhibited an increase in righting reflex time and a decrease in forelimb grip reflex time. Background EEG was significantly inhibited during HI induction and immediately after hypoxia and gradually recovered 72 h after hypoxia.
Seizures caused by HI brain damage in premature infants can be simulated in the P3 neonatal rat model.
围产期缺氧缺血性脑病（HIE）是早产儿癫痫和慢性神经功能障碍的主要原因。本研究旨在探讨HIE新生大鼠急性惊厥的特征及振幅整合脑电图（aEEG）背景活动模式。
通过结扎左侧颈总动脉并暴露于密闭缺氧环境2小时，诱导出生后第3天（P3）的新生大鼠（n = 12）发生缺氧缺血（HI）。收集惊厥类型、频率、持续时间以及神经行为发育相关数据，并分析脑电图背景的整合功率以评估HI的影响。
HI组所有新生大鼠在缺氧期间均频繁发生惊厥，83.3%的大鼠（10/12）在缺氧后立即发生惊厥。惊厥频率和持续时间随年龄增长逐渐降低。HI组的死亡率为8.33%（1/12）；HI诱导后120小时，仅27.3%（3/11）的幼崽分别出现低频和短持续时间的脑电图惊厥。HI损伤后96小时出现惊厥活动的HI大鼠，其翻正反射时间增加，前肢抓握反射时间减少。HI诱导期间和缺氧后立即脑电图背景受到显著抑制，缺氧后72小时逐渐恢复。
P3新生大鼠模型可模拟早产儿HI脑损伤所致惊厥。
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