Beijing Tongren Eye Center, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology & Visual Sciences Key Lab, Medical Artificial Intelligence Research and Verification Key Laboratory of the Ministry of Industry and Information Technology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Beijing Institute of Ophthalmology and Beijing Ophthalmology and Visual Science Key Lab, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
BMC Ophthalmol. 2022 Apr 27;22(1):193. doi: 10.1186/s12886-022-02417-8.
To examine an effect of intravitreally applied antibodies against epidermal growth factor family members, namely epiregulin, epigen and betacellulin, on ocular axial elongation.
The experimental study included 30 guinea pigs (age:3-4 weeks) which underwent bilateral lens-induced myopization and received three intraocular injections of 20 µg of epiregulin antibody, epigen antibody and betacellulin antibody in weekly intervals into their right eyes, and of phosphate-buffered saline into their left eyes. Seven days after the last injection, the animals were sacrificed. Axial length was measured by sonographic biometry.
At baseline, right eyes and left eyes did not differ (all P > 0.10) in axial length in neither group, nor did the interocular difference in axial length vary between the groups (P = 0.19). During the study period, right and left eyes elongated (P < 0.001) from 8.08 ± 0.07 mm to 8.59 ± 0.06 mm and from 8.08 ± 0.07 mm to 8.66 ± 0.07 mm, respectively. The interocular difference (left eye minus right eye) in axial elongation increased significantly in all three groups (epiregulin-antibody:from 0.03 ± 0.06 mm at one week after baseline to 0.16 ± 0.08 mm at three weeks after baseline;P = 0.001); epigen-antibody group:from -0.01 ± 0.06 mm to 0.06 ± 0.08 mm;P = 0.02; betacellulin antibody group:from -0.05 ± 0.05 mm to 0.02 ± 0.04 mm;P = 0.004). Correspondingly, interocular difference in axial length increased from -0.02 ± 0.04 mm to 0.13 ± 0.06 mm in the epiregulin-antibody group (P < 0.001), and from 0.01 ± 0.05 mm to 0.07 ± 0.05 mm in the epigen-antibody group (P = 0.045). In the betacellulin-antibody group the increase (0.01 ± 0.04 mm to 0.03 ± 0.03 mm) was not significant (P = 0.24).
The EGF family members epiregulin, epigen and betacellulin may be associated with axial elongation in young guinea pigs, with the effect decreasing from epiregulin to epigen and to betacellulin.
研究玻璃体内应用的表皮生长因子家族成员（即表皮调节素、上皮蛋白和β细胞素）的抗体对眼轴伸长的影响。
本实验研究包括 30 只 3-4 周龄的豚鼠，它们接受双侧晶状体诱导性近视，并在每周间隔内将 20μg 的表皮调节素抗体、上皮蛋白抗体和β细胞素抗体分别注入右眼，左眼注射磷酸盐缓冲盐水。最后一次注射后 7 天，处死动物。眼轴长度通过超声生物测量法测量。
在基线时，右眼和左眼在两组中（均 P>0.10）的眼轴长度没有差异，两组之间的眼轴长度的眼间差异也没有变化（P=0.19）。在研究期间，右眼和左眼从 8.08±0.07mm 分别延长到 8.59±0.06mm 和 8.08±0.07mm 到 8.66±0.07mm（P<0.001）。三组中右眼和左眼的眼轴伸长的眼间差异均显著增加（表皮调节素抗体：从基线后 1 周的 0.03±0.06mm 增加到基线后 3 周的 0.16±0.08mm；P=0.001）；上皮蛋白抗体组：从-0.01±0.06mm 增加到 0.06±0.08mm；P=0.02；β细胞素抗体组：从-0.05±0.05mm 增加到 0.02±0.04mm；P=0.004）。相应地，表皮调节素抗体组的眼间差异从 0.02±0.04mm 增加到 0.13±0.06mm（P<0.001），上皮蛋白抗体组从 0.01±0.05mm 增加到 0.07±0.05mm（P=0.045）。β细胞素抗体组的增加（0.01±0.04mm 至 0.03±0.03mm）不显著（P=0.24）。
表皮生长因子家族成员表皮调节素、上皮蛋白和β细胞素可能与幼年豚鼠的眼轴伸长有关，其作用从表皮调节素到上皮蛋白再到β细胞素逐渐减弱。
