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通过添加半胱氨酸去除甜瓜汁中的C9醛类和醇类物质。

Removal of C9-aldehydes and -alcohols from melon juice via cysteine addition.

作者信息

Aboshi Takako, Narita Konami, Katsumi Naoyuki, Ohta Tomoya, Murayama Tetsuya

机构信息

Faculty of Agriculture, Yamagata University, Tsuruoka, Japan.

Yamagata Integrated Agricultural Research Center, Minorigaoka, Yamagata, Japan.

出版信息

J Sci Food Agric. 2022 Oct;102(13):6131-6137. doi: 10.1002/jsfa.11965. Epub 2022 May 26.

Abstract

BACKGROUND

The aroma of a melon fruit is among the most crucial qualities that influence consumer preferences. However, strong grassy and cucumber-like aromas can prevent consumer acceptance. These grassy and cucumber-like aromas are produced by aldehydes containing nine-carbon chains. Several studies have revealed that aldehydes exhibit a high affinity toward cysteine. Thus, the effect of adding cysteine to volatile compounds to melon juice was investigated.

RESULTS

The volatile compounds released from the melon juice were analyzed via solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS) after 0.5, 5, and 24 h of adding cysteine. The results indicated that the concentrations of aldehydes, such as (E,Z)-2,6-nonadienal and (E)-2-nonenal, in the melon juice decreased after the addition of cysteine at all the analyzed times. Additionally, (E)-2-nonenol and (E,Z)-2,6-nonadien-1-ol, which were formed by the enzymatic reduction of the aldehydes, also decreased by cysteine addition. To confirm the binding of cysteine with the aldehydes, two cysteine adducts were analyzed via liquid chromatography-mass spectrometry (LC-MS) employing (E)-2-nonenal in the melon juice after the addition of cysteine.

CONCLUSION

This study demonstrates that cysteine addition could be potentially used to reduce the grassy and cucumber-like aromas of melon juice. © 2022 Society of Chemical Industry.

摘要

背景

甜瓜果实的香气是影响消费者偏好的最关键品质之一。然而,强烈的青草味和黄瓜味香气会阻碍消费者接受。这些青草味和黄瓜味香气是由含有九个碳链的醛类产生的。多项研究表明,醛类对半胱氨酸具有高亲和力。因此,研究了向甜瓜汁挥发性化合物中添加半胱氨酸的效果。

结果

在添加半胱氨酸0.5、5和24小时后,通过固相微萃取结合气相色谱-质谱联用(SPME-GC-MS)分析甜瓜汁释放的挥发性化合物。结果表明,在所有分析时间添加半胱氨酸后,甜瓜汁中(E,Z)-2,6-壬二烯醛和(E)-2-壬烯醛等醛类的浓度均降低。此外,由醛类酶促还原形成的(E)-2-壬烯醇和(E,Z)-2,6-壬二烯-1-醇,也因添加半胱氨酸而减少。为了证实半胱氨酸与醛类的结合,在添加半胱氨酸后,通过液相色谱-质谱联用(LC-MS)分析了甜瓜汁中两种半胱氨酸加合物,采用(E)-2-壬烯醛。

结论

本研究表明,添加半胱氨酸可能用于降低甜瓜汁的青草味和黄瓜味香气。©2022化学工业协会。

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