Tagrida Mohamed, Benjakul Soottawat
International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University Hat Yai Songkhla 90110 Thailand
RSC Adv. 2021 May 13;11(29):17630-17641. doi: 10.1039/d1ra02464g.
Different methods for chlorophyll removal were used for betel leaf ethanolic extracts (BLEE). Chlorophyll content, color, and antioxidant and antibacterial activities of the resulting extracts were examined. Sedimentation process remarkably reduced the chlorophyll content and color of BLEE ( < 0.05), while antioxidant and antibacterial activities were enhanced ( < 0.05). Polyphenol content and bioactivities of the extracts dechlorophyllized using organic solvents varied ( < 0.05). Antibacterial efficacy of BLEE dechlorophyllized by the sedimentation method (BLEE-SED) depended on concentrations. Lower minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of BLEE-SED toward 4 bacteria were obtained, compared to other extracts. Lower microbiological and chemical changes were achieved when Nile tilapia fillets were treated with BLEE-SED at 400 and 600 ppm after 12 days of storage at 4 °C. Therefore, sedimentation as a green process could be adopted for preparing a safe BLEE with augmented bioactivities and pale color, which could extend shelf-life of refrigerated Nile tilapia fillets.
对萎叶乙醇提取物（BLEE）采用了不同的叶绿素去除方法。检测了所得提取物的叶绿素含量、颜色以及抗氧化和抗菌活性。沉降过程显著降低了BLEE的叶绿素含量和颜色（P<0.05），同时增强了抗氧化和抗菌活性（P<0.05）。使用有机溶剂脱叶绿素的提取物的多酚含量和生物活性各不相同（P<0.05）。通过沉降法脱叶绿素的BLEE（BLEE-SED）的抗菌效果取决于浓度。与其他提取物相比，BLEE-SED对4种细菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）更低。在4℃储存12天后，用400和600 ppm的BLEE-SED处理尼罗罗非鱼鱼片时，微生物和化学变化更小。因此，沉降作为一种绿色工艺，可用于制备具有增强生物活性和浅色的安全BLEE，这可以延长冷藏尼罗罗非鱼鱼片的货架期。
