金雀异黄素联合维生素 C 作为一种新的辅助治疗胃癌的方法，可调节 ROS-STAT3 通路。
Piperlongumine combined with vitamin C as a new adjuvant therapy against gastric cancer regulates the ROS-STAT3 pathway.
机构信息
Institute of Basic Medical Sciences, Xi'an Medical University, Xi'an, Shaanxi Province, China.
China-Nepal Friendship Medical Research Centre of Rajiv Kumar Jha, School of Clinical Medicine, Xi'an Medical University, Xi'an, Shaanxi Province, China.
出版信息
J Int Med Res. 2022 Apr;50(4):3000605221093308. doi: 10.1177/03000605221093308.
OBJECTIVE
To investigate the effects of piperlongumine (PL) and vitamin C (VC) on signal transducer and activator of transcription 3 (STAT3) signalling in gastric cancer cell lines.
METHODS
tumour xenograft anticancer assays were undertaken to confirm the anticancer activity of PL. Cell viability, flow cytometry and Western blot assays were undertaken to evaluate the anticancer effects of PL, VC and combinations of PL and VC in AGS and KATO III cells.
RESULTS
Both PL and VC induced apoptosis and inhibited cell proliferation in AGS and KATO III cells. These effects were dependent on reactive oxygen species (ROS). PL effectively suppressed STAT3 activation while VC caused abnormal activation of STAT3. The combination of PL and VC exhibited a stronger apoptotic effect compared with either agent alone. PL reversed the abnormal activation of STAT3 by VC, which could be a key to their synergistic effect.
CONCLUSIONS
PL combined with VC exhibited a stronger anticancer effect by regulating the ROS-STAT3 pathway, suggesting that this combination might be a potential adjuvant therapy for gastric cancer.
目的
研究胡椒碱（PL）和维生素 C（VC）对胃癌细胞系信号转导和转录激活因子 3（STAT3）信号的影响。
方法
进行肿瘤异种移植抗癌测定以确认 PL 的抗癌活性。通过细胞活力、流式细胞术和 Western blot 测定评估 PL、VC 以及 PL 和 VC 联合在 AGS 和 KATO III 细胞中的抗癌作用。
结果
PL 和 VC 均诱导 AGS 和 KATO III 细胞凋亡并抑制细胞增殖。这些作用依赖于活性氧（ROS）。PL 可有效抑制 STAT3 激活，而 VC 导致 STAT3 异常激活。PL 和 VC 的联合使用比单独使用任一药物表现出更强的凋亡作用。PL 逆转了 VC 引起的 STAT3 异常激活，这可能是它们协同作用的关键。
结论
PL 联合 VC 通过调节 ROS-STAT3 通路表现出更强的抗癌作用，表明该联合可能是胃癌的一种潜在辅助治疗方法。
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