Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany.
Institute of Hygiene and Environmental Medicine, Charité - Universitätsmedizin Berlin, Berlin, Germany.
PLoS One. 2022 Apr 28;17(4):e0267669. doi: 10.1371/journal.pone.0267669. eCollection 2022.
Prompt pathogen identification of blood stream infections is essential to provide appropriate antibiotic treatment. Therefore, the objective of this prospective single centre study was to establish an inexpensive, fast and accurate protocol for bacterial species identification with SDS protein-extraction directly from BacT/Alert® blood culture (BC) bottles by VitekMS®.
Correct species identification was obtained for 198/266 (74.4%, 95%-CI = [68.8%, 79.6%]) of pathogens. The protocol was more successful in identifying 87/96 (91.4%, 95%-CI = [83.8%, 93.2%]) gram-negative bacteria than 110/167 (65.9%, 95%-CI = [58.1%, 73.0%]) gram-positive bacteria. The hands-on time for sample preparation and measurement was about 15 min for up to five samples. This is shorter than for most other protocols using a similar lysis-centrifugation approach for the combination of BacT/Alert® BC bottles and the Vitek® MS mass spectrometer. The estimated costs per sample were approx. 1.80€ which is much cheaper than for commercial kits.
This optimized protocol allows for accurate identification of bacteria directly from blood culture bottles for laboratories equipped with BacT/Alert® blood culture bottles and VitekMS® mass spectrometer.
快速鉴定血流感染的病原体对于提供恰当的抗生素治疗至关重要。因此，本前瞻性单中心研究的目的是建立一种经济、快速且准确的方案，通过 VitekMS® 直接从 BacT/Alert®血培养瓶（BC）中的 SDS 蛋白提取物中鉴定细菌种类。
198/266（74.4%，95%CI=[68.8%，79.6%]）的病原体得到了正确的种属鉴定。该方案在鉴定 87/96（91.4%，95%CI=[83.8%，93.2%]）革兰氏阴性菌方面比鉴定 110/167（65.9%，95%CI=[58.1%，73.0%]）革兰氏阳性菌更为成功。对于多达五个样本，样本制备和测量的实际操作时间约为 15 分钟。这比使用类似裂解离心方法的大多数其他方案用于 BacT/Alert®BC 瓶和 Vitek®MS 质谱仪的组合都要短。每个样本的估计成本约为 1.80 欧元，比商业试剂盒便宜得多。
对于配备有 BacT/Alert®血培养瓶和 VitekMS®质谱仪的实验室，本优化方案允许直接从血培养瓶中准确鉴定细菌。
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