Cineole alleviates the BPA-inhibited NETs formation by regulating the p38 pathway-mediated programmed cell death.

Bisphenol A (BPA) is an endocrine disruptor, that can cause immune dysfunction. Cineole (CIN) has that effect of regulating immune function and resist oxidation. Neutrophil extracellular traps (NETs) are one of the ways to resist pathogen invasion. In order to explore the effects of BPA and CIN on the release of chicken NETs and the antagonistic effect of CIN, take chicken peripheral blood neutrophils as object of study, grouping as NC, CIN, BPA + CIN and BPA. SEM, flow cytometry, RT-PCR, Western-blot and other methods were used to detect related indicators. The results showed that BPA inhibited the activities of GPX, SOD and CAT, increased the contents of MDA and NO, increased the activity of iNOS. BPA exposure inhibited the expression of myeloperoxidase (MPO), neutrophil elastase (NE) and histone, and inhibited the release of NETs. BPA activated downstream apoptosis and necroptosis through the p38 mitogen-activated protein kinase (p38-MAPK) pathway, which increased the expression of cytochrome C (CytC), bcl-2 associated K protein gene (bak), cysteinyl aspartate specific proteinase 3 (caspase-3), cysteinyl aspartate specific proteinase 9 (caspase-9), receptor-interacting protein kinase 1 (RIPK1), receptor-interacting protein kinase 1 (RIPK3) and mixed lineage kinase domain-like protein (MLKL), decreased the expression of B-cell lymphoma-2 (bcl-2). However, the co-exposure of CIN and BPA partially recovered the release of NETs, alleviated BPA-induced oxidative stress, and inhibited the activation of p38-MAPK pathway, necroptosis, and mitochondrial apoptosis pathway. These results indicated that CIN modulated p38 pathway alleviated BPA-induced neutrophil necroptosis and apoptosis, and increased NETs formation.