University of Florida, Department of Plant Pathology, PO Box 110680, Gainesville, FL, 32611-0180, United States.
University of Florida, Department of Plant Pathology, PO Box 110680, Gainesville, FL, 32611-0180, United States.
Fungal Biol. 2022 May;126(5):342-355. doi: 10.1016/j.funbio.2022.03.004. Epub 2022 Mar 28.
Stem blight is a major disease of blueberry caused by Botryosphaeriaceae fungi. Chemical and cultural management options are limited, putting emphasis on breeding efforts to identify sources of resistance. The efficacy and durability of host resistance could be impacted by the species composition of the pathogen population in a region and by the isolates employed in the screenings used to identify the resistance. Samples (365) were collected from southern highbush (SHB) and rabbiteye blueberry (REB) cultivars from 28 sites in the southeastern US (AL, FL, GA, NC, and SC). Colony morphology identified 86% of the isolates as Botryosphaeriaceae. Conidia morphology and Maximum Likelihood analysis of the Internal Transcribed Spacer rDNA regions (ITS), translation elongation factor one alpha (tef1-α), and β-tubulin were used to identify isolates at genera or species level. A PCR-restriction fragment length polymorphism (PCR-RFLP) test was used to identify isolates to genus. Neofusicoccum and Lasiodiplodia were the predominant genera. N. kwambonambiense, N. ribis, L. theobromae and L. pseudotheobromae were the most common species isolated. Phylogenies conducted with a limited number of isolates indicated non-clonal and potentially diverse populations occur on blueberry that warrant additional study. Botryosphaeria corticis, B. dothidea, and Diplodia seriata were isolated infrequently.
茎枯病是一种由 Botryosphaeriaceae 真菌引起的蓝莓主要病害。化学和文化管理选项有限，因此强调了培育工作以确定抗性来源。宿主抗性的功效和耐久性可能会受到该地区病原菌种群的物种组成以及用于筛选鉴定抗性的分离物的影响。从美国东南部（阿拉巴马州、佛罗里达州、佐治亚州、北卡罗来纳州和南卡罗来纳州）的 28 个地点采集了南方高丛蓝莓（SHB）和兔眼蓝莓（REB）品种的 365 个样本。菌落形态学将 86%的分离物鉴定为 Botryosphaeriaceae。分生孢子形态和内转录间隔区 rDNA 区（ITS）、翻译延伸因子 1α（tef1-α）和β-微管蛋白的最大似然分析用于在属或种水平上鉴定分离物。聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）测试用于鉴定属的分离物。Neofusicoccum 和 Lasiodiplodia 是主要的属。分离出的 N. kwambonambiense、N. ribis、L. theobromae 和 L. pseudotheobromae 是最常见的物种。对少数分离物进行的系统发育分析表明，蓝莓上存在非克隆和潜在多样化的种群，这需要进一步研究。Botryosphaeria corticis、B. dothidea 和 Diplodia seriata 分离频率较低。
