非肥胖墨西哥成年人的维生素D受体基因多态性、β细胞功能及维生素D状态

Vitamin D Receptor Gene Polymorphisms, β-cell Function, and Vitamin D Status in Non-obese Mexican Adults.

作者信息

Piña-Aguero Mónica Ivette, Maldonado-Hernández Jorge, Sebastián-Medina Leticia, Tejero-Barrera María Elizabeth, Robledo-Pérez Ricardo Martín, Villalpando-Hernández Salvador, Ventura-Bravo Zobeida Analilia, Morales-Ramírez Laura Katia

机构信息

Unidad de Investigación Médica en Nutrición, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México.

出版信息

Arch Med Res. 2022 Jun;53(4):416-422. doi: 10.1016/j.arcmed.2022.04.002. Epub 2022 Apr 29.

DOI:10.1016/j.arcmed.2022.04.002

PMID:35501225

Abstract

BACKGROUND

FokI (rs2228570 T>C) and BsmI (rs1544410 A>G) polymorphisms of the vitamin D receptor (VDR) have been associated to abnormal glucose metabolism and could be inversely associated with β-cell function (BCF) and vitamin D status. There is a lack of information about this topic in the Mexican population.

AIM OF THE STUDY

To evaluate the relationship between VDR gene polymorphisms FokI and BsmI with BCF and vitamin D status in a population of non-obese Mexican adults.

METHODS

A sample of 192 participants were enrolled during 2016-2018. Blood samples were collected to determine fasting concentrations of glucose, insulin, and vitamin D. Genomic DNA was isolated from leucocytes and the polymorphic variants of FokI and BsmI were analyzed. The Homeostasis Model Assessment Calculator was used to estimate the BCF (HOMA2-B).

RESULTS

FokI polymorphism showed a frequency of 20.1% for homozygous TT carriers and 7.8% for the BsmI GG. The recessive model of FokI (TT genotype) showed a lower mean value of BCF compared to the combination of CC + CT (99.2 vs. 109.6%, p = 0.045). Likewise, significantly lower mean values of HOMA2-B and insulin were observed for BsmI (GG genotype, p = 0.016 and p = 0.039, respectively). After covariates adjustment, only FokI polymorphism remained as an independent predictor of BCF.

CONCLUSIONS

the TT and GG variants of the FokI and BsmI polymorphisms are related to a decrease in FCB. In the case of FokI, this decrement was independent of insulin sensitivity, vitamin D levels, percentage of body fat, gender, and age.

摘要

背景

维生素D受体（VDR）的FokI（rs2228570 T>C）和BsmI（rs1544410 A>G）基因多态性与葡萄糖代谢异常有关，可能与β细胞功能（BCF）和维生素D状态呈负相关。墨西哥人群中缺乏关于该主题的信息。

研究目的

评估非肥胖墨西哥成年人中VDR基因多态性FokI和BsmI与BCF及维生素D状态之间的关系。

方法

2016年至2018年招募了192名参与者作为样本。采集血样以测定空腹血糖、胰岛素和维生素D浓度。从白细胞中分离基因组DNA，并分析FokI和BsmI的多态性变体。使用稳态模型评估计算器估算BCF（HOMA2-B）。

结果

FokI基因多态性中纯合子TT携带者的频率为20.1%，BsmI GG为7.8%。与CC + CT组合相比，FokI的隐性模型（TT基因型）显示BCF的平均值较低（99.2%对109.6%，p = 0.045）。同样，BsmI（GG基因型）的HOMA2-B和胰岛素平均值显著较低（分别为p = 0.016和p = 0.039）。在调整协变量后，只有FokI基因多态性仍然是BCF的独立预测因子。