Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China; Bio-Innovation Center of DR PLANT, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
Phytochemistry. 2022 Aug;200:113223. doi: 10.1016/j.phytochem.2022.113223. Epub 2022 May 3.
Dendrobium officinale Kimura et Migo, one of the most important orchids because of its medicinal and edible value, has a typical Dendrobium Sw. flora scent, which has great application potential and commercial value to be characterized. The aroma-active compounds originating from D. officinale fresh flowers (DFF) were investigated using a sensomics approach. A combined solid phase microextraction and solvent-assisted flavor evaporation method were used to accurately capture the overall aromatic profile. Exactly 34 odorants were detected and identified by aroma extract dilution analysis (AEDA) coupled with gas chromatography/olfactometry-mass spectrometry (GC/O-MS) in DFF, of which nine odorants had a flavor dilution (FD) factor ≥27. All 34 odorants were further quantified. The odor activity values (OAVs) were calculated with the highest value of 7444, in which 18 compounds were confirmed to be key odorants, including 1-octen-3-ol, hexanal, nonanal, phenylacetaldehyde, linalool, 4-oxoisophorone, theaspirane, methyl salicylate, etc. Among the studies above, 42 out of 78 volatiles and 14 out of 34 odorants were identified in DFF for the first time. Then, the aroma profile of the DFF was simulated successfully by aroma recombination experiments based on the quantitation results, and the omission test suggested that alcohols are the decisive type of compounds in the DFF key odorants. In addition, a progressive addition test showed that the aroma recombinate prepared with 18 reference key odorants was able to reconstruct the characteristic aroma of DFF. In comparison, the recombinate constituted by mixing all 34 reference odorants in the same concentrations as determined in the DDF sample could mimic the flower scent and closely match the sensory attributes of the original D. officinale fresh flower.
铁皮石斛，因其药用和食用价值而成为最重要的兰花之一，具有典型的石斛属植物香气，具有很大的应用潜力和商业价值有待进一步研究。采用感观组学方法研究了铁皮石斛鲜花（DFF）中的香气活性化合物。采用固相微萃取和溶剂辅助风味蒸发相结合的方法，准确地捕捉了整体香气轮廓。通过香气提取物稀释分析（AEDA）与气相色谱/嗅觉测量-质谱（GC/O-MS）相结合，在 DFF 中共检测到并鉴定了 34 种气味物质，其中 9 种气味物质的风味稀释（FD）因子≥27。对 34 种气味物质进行了进一步的定量分析。用最高值 7444 计算了气味活性值（OAV），其中 18 种化合物被确认为关键气味物质，包括 1-辛烯-3-醇、己醛、壬醛、苯乙醛、芳樟醇、4-氧异佛尔酮、天门冬酰胺、水杨酸甲酯等。在上述研究中，DFF 中首次鉴定出 78 种挥发物中的 42 种和 34 种气味物质中的 14 种。然后，基于定量结果，通过香气重组实验成功模拟了 DFF 的香气特征，排除试验表明醇类是 DFF 关键气味物质中的决定性化合物类型。此外，逐步添加试验表明，用 18 种参考关键气味物质制备的香气重组物能够重建 DFF 的特征香气。相比之下，由混合所有 34 种参考气味物质以与 DDF 样品中相同浓度组成的重组物可以模拟花香，并与原始铁皮石斛鲜花的感官属性非常匹配。
