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面向所有人的鱼类原位杂交技术：一种用于海洋胚胎和幼体的快速高效荧光杂交（FISH）方案

FISH for All: A Fast and Efficient Fluorescent Hybridization (FISH) Protocol for Marine Embryos and Larvae.

作者信息

Paganos Periklis, Caccavale Filomena, La Vecchia Claudia, D'Aniello Enrico, D'Aniello Salvatore, Arnone Maria Ina

机构信息

Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Naples, Italy.

出版信息

Front Physiol. 2022 Apr 19;13:878062. doi: 10.3389/fphys.2022.878062. eCollection 2022.

DOI:10.3389/fphys.2022.878062
PMID:35514360
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9062072/
Abstract

hybridization is one the most commonly used techniques for developmental and evolutionary biology and has extensively contributed to the identification of distinct cell types and cell states, as well dissecting several molecular mechanisms involved in physiological processes. Moreover, it has been used as a tool to compare distinct gene expression patterns and, therefore, genetic programs across animal species. Nowadays, the predominance of transcriptomics in science has imposed the need to establish a reliable, fast and easy whole mount hybridization protocol. Here we describe a fluorescent hybridization protocol that is rapid, accurate and applicable in a great variety of marine species.

摘要

杂交是发育生物学和进化生物学中最常用的技术之一，它为识别不同的细胞类型和细胞状态做出了广泛贡献，同时也有助于剖析生理过程中涉及的多种分子机制。此外，它还被用作比较不同动物物种基因表达模式以及遗传程序的工具。如今，转录组学在科学领域占据主导地位，这就需要建立一种可靠、快速且简便的全组织杂交方案。在此，我们描述一种荧光杂交方案，该方案快速、准确且适用于多种海洋物种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/2e04f658323f/fphys-13-878062-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/a288fb9885f9/fphys-13-878062-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/f79d1ab4a82a/fphys-13-878062-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/d2ec38763a9a/fphys-13-878062-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/2e04f658323f/fphys-13-878062-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/a288fb9885f9/fphys-13-878062-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/f79d1ab4a82a/fphys-13-878062-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/d2ec38763a9a/fphys-13-878062-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/9062072/2e04f658323f/fphys-13-878062-g004.jpg

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