Fang Guiqian, Wang Hao, Bian Zhancun, Guo Min, Wu Zhongyu, Yao Qingqiang
School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences Jinan 250200 Shandong China
Institute of Materia Medica, Shandong Academy of Medical Sciences Jinan 250062 Shandong China.
RSC Adv. 2019 Jun 28;9(35):20306-20313. doi: 10.1039/c9ra03978c. eCollection 2019 Jun 25.
Boronic acid provides faster fluorescence response to Fe compared to other reported sensors, which is critical for continuous dynamic detection. Herein, we reported a novel boronic acid-based sensor 4 that could recognize Fe ion in real time. After 10 equiv. of Fe ion (1 mM) was added, the fluorescence of sensor 4 was immediately quenched by 96%. While other ions, including Ba, Ca, Cr, Cd, Co, Cs, Cu, Fe, K, Li, Mg, Mn, Na, Ni or Zn, respectively, did not change the fluorescence significantly. Further tests indicated that the high selectively sensing Fe ion benefits from the two boronic acid functionalities in the structure. Moreover, interference experiments showed this sensor has an excellent anti-interference ability. In addition, we performed binding activity test in rabbit plasma and other real samples for practical applications, obtaining similar results. And the thin layer loading sensor 4 was also successfully applied to recognize Fe ion among various ions. Therefore, 4 may serve as a potential sensor for continuous monitoring and detecting Fe ion in real time.
与其他已报道的传感器相比，硼酸对铁具有更快的荧光响应，这对于连续动态检测至关重要。在此，我们报道了一种新型的基于硼酸的传感器4，它能够实时识别铁离子。加入10当量的铁离子（1 mM）后，传感器4的荧光立即猝灭了96%。而其他离子，包括钡、钙、铬、镉、钴、铯、铜、铁、钾、锂、镁、锰、钠、镍或锌，分别加入时，荧光没有明显变化。进一步的测试表明，对铁离子的高选择性传感得益于结构中的两个硼酸官能团。此外，干扰实验表明该传感器具有出色的抗干扰能力。此外，我们在兔血浆和其他实际样品中进行了结合活性测试以用于实际应用，获得了相似的结果。并且薄层负载的传感器4也成功地应用于在各种离子中识别铁离子。因此，4可能作为一种潜在的传感器用于实时连续监测和检测铁离子。
