CgHMGB1 作为一种广谱识别分子，通过 MAPK 或 NF-κB 信号通路诱导虾夷扇贝 CgIL17-5 和 Cgdefh2 的表达。
CgHMGB1 functions as a broad-spectrum recognition molecule to induce the expressions of CgIL17-5 and Cgdefh2 via MAPK or NF-κB signaling pathway in Crassostrea gigas.
机构信息
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai 519082, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China.
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai 519082, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China.
出版信息
Int J Biol Macromol. 2022 Jun 30;211:289-300. doi: 10.1016/j.ijbiomac.2022.04.166. Epub 2022 May 4.
High-mobility group box 1 (HMGB1), a highly conserved nucleoprotein, functions in immune recognition, inflammation and antibacterial immunization in vertebrates. In the present study, the mediation mechanism of CgHMGB1 in activating MAPK and NF-κB/Rel signaling pathways to induce the expressions of immune effectors was investigated. CgHMGB1 mRNA was detected in all tested developmental stages from fertilized egg to D-larvae, with the higher expressions in 4 cells and 8 cells stages. CgHMGB1 proteins were mainly distributed in haemocyte granulocytes. The expressions of CgHMGB1 mRNA in haemocytes increased significantly after Vibrio splendidus stimulation, and CgHMGB1 protein translocated into the haemocyte cytoplasm and release into cell-free haemolymph. The phosphorylation of CgERK and CgP38 were induced, the nuclear translocation of CgRel were promoted, and the mRNA expressions of CgIL17-5 and Cgdefh2 increased significantly after rCgHMGB1 treatment. Obvious branchial swelling and cilium shedding were observed after rCgHMGB1 treatment. rCgHMGB1 exhibited binding activity to different polysaccharides, bacteria, and fungi. rCgHMGB1 also displayed obvious antibacterial activity to V. splendidus and E. coli. These results indicated that CgHMGB1 functioned as an immune recognition molecule to recognize various PAMPs and bacteria to induce the mRNA expressions of CgIL17-5 and Cgdefh2 via the activation of MAPK and NF-κB signaling pathways in oysters.
高迁移率族蛋白 B1（HMGB1）是一种高度保守的核蛋白，在脊椎动物的免疫识别、炎症和抗菌免疫中发挥作用。本研究探讨了 CgHMGB1 介导 MAPK 和 NF-κB/Rel 信号通路激活，诱导免疫效应物表达的机制。从受精卵到 D 期幼虫的所有发育阶段均检测到 CgHMGB1mRNA 的表达，其中在 4 细胞和 8 细胞阶段表达较高。CgHMGB1 蛋白主要分布在血细胞的粒细胞中。在受到灿烂弧菌刺激后，血细胞中 CgHMGB1mRNA 的表达显著增加，CgHMGB1 蛋白易位到血细胞细胞质并释放到无细胞的血淋巴中。rCgHMGB1 处理后，CgERK 和 CgP38 的磷酸化被诱导，CgRel 核转位被促进，CgIL17-5 和 Cgdefh2 的 mRNA 表达显著增加。rCgHMGB1 处理后，出现明显的鳃肿胀和纤毛脱落。rCgHMGB1 与不同的多糖、细菌和真菌具有结合活性。rCgHMGB1 对灿烂弧菌和大肠杆菌也表现出明显的抗菌活性。这些结果表明，CgHMGB1 作为一种免疫识别分子，通过激活 MAPK 和 NF-κB 信号通路，识别各种 PAMPs 和细菌，诱导牡蛎 CgIL17-5 和 Cgdefh2 的 mRNA 表达。
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