College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China; Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, PR China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control (Huazhong Agricultural University), Wuhan, Hubei, PR China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China; Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, PR China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control (Huazhong Agricultural University), Wuhan, Hubei, PR China.
Food Chem. 2022 Sep 30;389:133130. doi: 10.1016/j.foodchem.2022.133130. Epub 2022 May 2.
Low methoxyl citrus pectin (LMP) de-esterified from high hydrostatic pressure assisted enzymatic (HHP-pectin) and two other traditional methods was characterized, and curcumin-pectin calcium gel beads were prepared to study curcumin-loading and colon-targeted delivery abilities. It was found there was no significant difference among different LMP on amorphous structure, while significantly higher Rha/GalA ratio of HHP-pectin indicated higher rhamnogalacturonan Ⅰ (RG-I) proportion and branching extent. Curcumin was well embedded in LMP beads with improved stability. HHP-pectin beads showed significantly higher hardness, chewiness and encapsulation efficiency, while lower swelling ratio. Moreover, in vitro simulated digestion showed gel beads can deliver curcumin to colon and inhibit premature release. HHP-pectin beads could release curcumin more quickly in colon, probably because of differences on texture properties, which may be depended on molecular structure. Thus, LMP especially HHP-pectin calcium gel as colon-targeted delivery system for curcumin may have potential application in function food and drug delivery.
从高静压辅助酶解（HHP-pectin）和另外两种传统方法中提取的低甲氧基柑橘果胶（LMP）进行了特性分析，并制备了姜黄素-果胶钙凝胶珠，以研究姜黄素的负载和结肠靶向递送能力。结果表明，不同 LMP 在无定形结构方面没有显著差异，而 HHP-pectin 中 Rha/GalA 比值显著较高，表明 RG-I 比例和支化程度更高。姜黄素很好地嵌入 LMP 珠中，稳定性提高。HHP-pectin 珠表现出更高的硬度、韧性和包封效率，而膨胀率更低。此外，体外模拟消化表明，凝胶珠可以将姜黄素递送到结肠并抑制过早释放。HHP-pectin 珠在结肠中能更快地释放姜黄素，可能是因为在质地特性上存在差异，这可能取决于分子结构。因此，LMP 特别是 HHP-pectin 钙凝胶作为姜黄素的结肠靶向递送系统可能在功能性食品和药物递送方面具有潜在的应用。
