Lisowsky T, Schweizer E, Michaelis G
Eur J Biochem. 1987 May 4;164(3):559-63. doi: 10.1111/j.1432-1033.1987.tb11163.x.
Mitochondrial transcription was studied in a nuclear temperature-sensitive pet mutant of Saccharomyces cerevisiae. The mitochondrial RNA levels in vivo and the in vitro transcriptional activities of isolated mitochondria were analysed. In comparison to the wild-type an overall reduction of mitochondrial gene expression together with a changed expression pattern was observed for the mutant, indicating a defect in mitochondrial RNA synthesis. These findings were supported by studies with a purified DNA-protein complex from yeast mitochondria. This complex was able to synthesize ribosomal and messenger RNAs in an in vitro system. Proteins from wild-type and mutant transcription complexes were tested for their DNA-binding abilities; one of the proteins identified in the wild type had either lost this ability or was absent in the mutant.
在酿酒酵母的一个核温度敏感型pet突变体中研究了线粒体转录。分析了体内线粒体RNA水平以及分离线粒体的体外转录活性。与野生型相比，观察到该突变体线粒体基因表达总体降低且表达模式改变，表明线粒体RNA合成存在缺陷。来自酵母线粒体的纯化DNA-蛋白质复合物的研究支持了这些发现。该复合物能够在体外系统中合成核糖体RNA和信使RNA。测试了野生型和突变型转录复合物中的蛋白质的DNA结合能力；在野生型中鉴定出的一种蛋白质在突变体中要么失去了这种能力，要么不存在。
