Wang Wen-Fang, Wang Xu-Song, Tan San-Yang, Zhong Lan-Lan, Chen Jiang
Department of Clinical Laboratory, Haikou Maternal and Child Health Hospital, Haikou 570100, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2022 Apr 15;24(4):411-416. doi: 10.7499/j.issn.1008-8830.2110139.
To study the expression level of plasma miR-106b-5p in primary immune thrombocytopenia (ITP) and its correlation with the levels of T helper 17 cell (Th17) and regulatory T cell (Treg) and the Th17/Treg ratio.
A total of 79 children with ITP (ITP group) and 40 healthy children (control group) were selected as subjects. According to the treatment response, the 79 children with ITP were divided into three groups: complete response (=40), partial response (=18), and non-response (=21). Quantitative real-time PCR was used to measure the expression level of miR-106b-5p. Flow cytometry was used to measure the frequencies of Th17 and Treg, and the Th17/Treg ratio was calculated. The correlation of the expression level of plasma miR-106b-5p with the frequencies of Th17 and Treg and the Th17/Treg ratio was analyzed.
Compared with the control group, the ITP group had significantly higher levels of miR-106b-5p, Th17, and Th17/Treg ratio (<0.05) and a significantly lower level of Treg (<0.05). After treatment, the ITP group had significant reductions in the levels of miR-106b-5p, Th17, and Th17/Treg ratio (<0.05) and a significant increase in the level of Treg (<0.05). Compared with the partial response and non-response groups, the complete response group had significantly lower levels of miR-106b-5p, Th17, and Th17/Treg ratio (<0.05) and a significantly higher level of Treg (<0.05). The correlation analysis showed that in the children with ITP, the expression level of plasma miR-106b-5p was positively correlated with the Th17 level and the Th17/Treg ratio (=0.730 and 0.816 respectively; <0.001) and was negatively correlated with the Treg level (=-0.774, <0.001).
A higher expression level of miR-106b-5p and Th17/Treg imbalance may be observed in children with ITP. The measurement of miR-106b-5p, Th17, Treg, and Th17/Treg ratio during treatment may be useful to the evaluation of treatment outcome in children with ITP.
研究原发性免疫性血小板减少症（ITP）患儿血浆miR-106b-5p的表达水平及其与辅助性T细胞17（Th17）、调节性T细胞（Treg）水平及Th17/Treg比值的相关性。
选取79例ITP患儿（ITP组）和40例健康儿童（对照组）作为研究对象。根据治疗反应，将79例ITP患儿分为三组：完全缓解组（n = 40）、部分缓解组（n = 18）和未缓解组（n = 21）。采用定量实时荧光定量PCR检测miR-106b-5p的表达水平。采用流式细胞术检测Th17和Treg的频率，并计算Th17/Treg比值。分析血浆miR-106b-5p表达水平与Th17、Treg频率及Th17/Treg比值的相关性。
与对照组相比，ITP组miR-106b-5p、Th17水平及Th17/Treg比值显著升高（P < 0.05），Treg水平显著降低（P < 0.05）。治疗后，ITP组miR-106b-5p、Th17水平及Th17/Treg比值显著降低（P < 0.05），Treg水平显著升高（P < 0.05）。与部分缓解组和未缓解组相比，完全缓解组miR-106b-5p、Th17水平及Th17/Treg比值显著降低（P < 0.05），Treg水平显著升高（P < 0.05）。相关性分析显示，ITP患儿血浆miR-106b-5p表达水平与Th17水平及Th17/Treg比值呈正相关（r分别为0.730和0.816；P < 0.001），与Treg水平呈负相关（r = -0.774，P < 0.001）。
ITP患儿可能存在miR-106b-5p表达水平升高及Th17/Treg失衡。治疗过程中检测miR-106b-5p、Th17、Treg及Th17/Treg比值可能有助于评估ITP患儿的治疗效果。
