Chen Yun, Wang Kai, Zhang Shouquan
Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512000, China.
National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
J Anim Sci Technol. 2022 Mar;64(2):235-246. doi: 10.5187/jast.2022.e15. Epub 2022 Mar 31.
In this study, we used more reliable experimental materials and methods to detect the effects of osteopontin (OPN) on boar sperm capacitation, acrosome reaction, and fertilization efficiency. We reorganized and obtained the OPN protein of the porcine source. Immunofluorescence and Western blot show the localization and expression of the OPN protein before and after sperm capacitation. To determine whether OPN can affect sperm during sperm capacitation, we examined cyclic adenosine monophosphate (cAMP) concentrations after sperm capacitation, and the results showed that OPN significantly increased the cAMP concentration in sperm ( < 0.05). Flow cytometry showed that 0.1 μg/mL OPN-treated sperm had better acrosome reaction ability. fertilization (IVF) showed that 0.1 μg/mL OPN significantly increased the rate of embryo division. In conclusion, this study found that 0.1 μg/mL porcine OPN protein can significantly improve porcine capacitated sperm motility, cAMP concentration after capacitation sperm, acrosome reaction ability, and embryo division during IVF and provides new clues to explore the mechanism of OPN's function on sperm.
在本研究中，我们使用了更可靠的实验材料和方法来检测骨桥蛋白（OPN）对公猪精子获能、顶体反应和受精效率的影响。我们重组并获得了猪源OPN蛋白。免疫荧光和蛋白质免疫印迹显示了精子获能前后OPN蛋白的定位和表达。为了确定OPN在精子获能过程中是否会影响精子，我们检测了精子获能后的环磷酸腺苷（cAMP）浓度，结果显示OPN显著提高了精子中的cAMP浓度（<0.05）。流式细胞术显示，经0.1μg/mL OPN处理的精子具有更好的顶体反应能力。体外受精（IVF）显示，0.1μg/mL OPN显著提高了胚胎分裂率。总之，本研究发现0.1μg/mL猪OPN蛋白可显著提高猪获能精子活力、获能精子后的cAMP浓度、顶体反应能力以及IVF过程中的胚胎分裂率，并为探索OPN对精子作用机制提供了新线索。
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