Feng Guangxia, He Qinghua, Xie WenYue, He Yonghong, Chen Xuejing, Wang Bei, Lu Bangrong, Guan Tian
Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University Shenzhen 518055 People's Republic of China
School of Medicine, Tsinghua University Beijing 100084 People's Republic of China.
RSC Adv. 2018 Jun 11;8(38):21272-21279. doi: 10.1039/c8ra02410c. eCollection 2018 Jun 8.
The rapid growth of demand for high-throughput multiplexed biochips from modern biotechnology has led to growing interest in suspension array based on multi-channel encoded microbeads. We prepare dual-spectra encoded PEGDA microbeads (DSEPM) by reversed-phase microemulsion UV curing method and layer-by-layer electrostatic self-assembly method. Excitation of the synthesized DSEPM results in two spectra, including fluorescence spectra from quantum dots and laser induced breakdown spectra from nanoparticles with specific elements. With further surface modification and bio-probes grafting, we use DSEPM to carry a series of detection experiments of biomolecules. The adsorption experiment to two types of anti-IgG in mixture sample has demonstrated the availability of DSEPM in multiplexing. Then, the contrast experiment has verified the specificity of DSEPM in detection. Finally, we carry out the concentration gradient experiment and obtain the response curve to show the performance of DSEPM in quantitative analysis. The results indicate our method provide an effective way to improve multiplexed biochips with more coding capacity, accuracy and stability.
现代生物技术对高通量多重生物芯片需求的快速增长,引发了人们对基于多通道编码微珠的悬浮阵列的日益浓厚兴趣。我们通过反相微乳液紫外固化法和层层静电自组装法制备了双光谱编码聚乙二醇二丙烯酸酯微珠(DSEPM)。合成的DSEPM激发后会产生两种光谱,包括量子点的荧光光谱和具有特定元素的纳米颗粒的激光诱导击穿光谱。通过进一步的表面修饰和生物探针接枝,我们使用DSEPM进行了一系列生物分子检测实验。对混合样品中两种抗IgG的吸附实验证明了DSEPM在多重检测中的可用性。然后,对比实验验证了DSEPM在检测中的特异性。最后,我们进行了浓度梯度实验并获得了响应曲线,以展示DSEPM在定量分析中的性能。结果表明,我们的方法为改进具有更多编码能力、准确性和稳定性的多重生物芯片提供了一种有效途径。