Yang Fang, Huang Xingjian, Zhang Conglan, Zhang Mei, Huang Chao, Yang Hao
Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology Wuhan 430205 China
Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University Wuhan 430070 China.
RSC Adv. 2018 Jan 10;8(5):2653-2659. doi: 10.1039/c7ra13007d. eCollection 2018 Jan 9.
The amino acid composition, nutritional value and proteins subunit of chestnuts ( Blume) from three regions of China (Henan, Hunan, and Guangdong) were investigated. Experimental results showed that the albumin fraction dominated the chestnut protein composition, but globulin and prolamin were nondetectable. All the Chinese chestnut proteins had a nutritionally balanced amino acid composition. Their amino acid score (AAS) could reach the FAO/WHO (2013) requirement for essential amino acids for older children, adolescents and adults. Leucine was the first limiting amino acid for all the Chinese chestnut protein isolates and digestible indispensable amino acid scores (DIAAS) were more than 90 (Leu). The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) spectra showed that different Chinese chestnut protein isolates had almost the same band components, which were mainly comprised of seven polypeptide segments with the molecular weights of (91-93) kDa, (70-72) kDa, (53-55) kDa, 37 kDa, (27-33) kDa, 20 kDa and (5-15) kDa. The amino acid compositions and the abundance of low molecular weight proteins indicated that Chinese chestnut could be utilized as a good source of plant protein for human nutrition.
对来自中国三个地区（河南、湖南和广东）的板栗（Blume）的氨基酸组成、营养价值和蛋白质亚基进行了研究。实验结果表明，清蛋白部分在板栗蛋白质组成中占主导地位，但未检测到球蛋白和醇溶蛋白。所有中国板栗蛋白质的氨基酸组成营养均衡。它们的氨基酸评分（AAS）能够达到粮农组织/世界卫生组织（2013年）对大龄儿童、青少年和成年人必需氨基酸的要求。亮氨酸是所有中国板栗蛋白质分离物的第一限制氨基酸，可消化必需氨基酸评分（DIAAS）均超过90（以亮氨酸计）。十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）图谱显示，不同的中国板栗蛋白质分离物具有几乎相同的条带成分，主要由七条多肽片段组成，分子量分别为（91 - 93）kDa、（70 - 72）kDa、（53 - 55）kDa、37 kDa、（27 - 33）kDa、20 kDa和（5 - 15）kDa。氨基酸组成和低分子量蛋白质的丰度表明，中国板栗可作为人类营养的优质植物蛋白来源。
