State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China.
Exp Suppl. 2022;114:137-152. doi: 10.1007/978-3-030-93306-7_6.
The microsporidium Nosema bombycis can infect and transmit both vertically and horizontally in multiple lepidopteran insects including silkworms and crop pests. While there have been several studies on the N. bombycis spore, there have been only limited studies on the N. bombycis sporoplasm. This chapter reviews what is known about this life cycle stage as well as published studies on purification of the N. bombycis sporoplasm and its survival in an in vitro cell culture system. Genetic transformation techniques have revolutionized the study of many pathogenic organisms. While progress has been made on the development of such systems for microsporidia, this critical problem has not been solved for these pathogens. This chapter provides a summary of the latest research progress on genetic manipulation of N. bombycis.
微孢子虫 Nosema bombycis 可以在包括家蚕和农作物害虫在内的多种鳞翅目昆虫中垂直和水平传播。虽然已经有几项关于 N. bombycis 孢子的研究，但对 N. bombycis 孢质的研究却非常有限。本章回顾了这一生命周期阶段的已知情况，以及关于 N. bombycis 孢质的纯化及其在体外细胞培养系统中存活的已发表研究。遗传转化技术彻底改变了许多病原生物的研究。虽然已经在开发用于微孢子虫的这类系统方面取得了进展，但这些病原体的这一关键问题尚未得到解决。本章总结了 N. bombycis 遗传操作的最新研究进展。
