Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran.
Department of Venomous Animals and Anti Venom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Arch Razi Inst. 2021 Dec 30;76(6):1727-1733. doi: 10.22092/ARI.2021.126291.1342. eCollection 2021 Dec.
Experimental autoimmune encephalomyelitis (EAE) has been widely employed as a model to study multiple sclerosis (MS). Interleukin-27 (IL-27) inhibits Th17 activity and breaks the normal activity of effector T cells which cause autoimmunity. Bee venom (BV) has been used as a form of medicine from the time of ancient Greece and China. BV and BV-derived active components might have potent therapeutic effects on refractory immunological and neurodegenerative diseases, such as MS. This study aimed to investigate the effect of Iranian honey bee venom on the progression of EAE in mice. Initially, EAE was induced in 12 female C57BL/6 mice through immunization with an emulsion of myelin oligodendrocyte glycoprotein 35-55 (MOG) in Complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTx) in phosphate buffer. Following the appearance of clinical signs, the mice were treated intraperitoneally with BV. Histopathological and immunological studies were investigated, and EAE was induced in animals within 9-14 days. Results revealed a significant reduction in IL-27 levels following EAE induction in mice. However, BV-treated mice showed a significant increase in IL-27, compared to controls. Histopathology results revealed that the number of inflammatory cells was reduced in the brain parenchyma following BV treatment. Based on the results obtained in the present study, BV may be a suitable candidate for the treatment of inflammatory diseases, such as MS.
实验性自身免疫性脑脊髓炎 (EAE) 已被广泛用作研究多发性硬化症 (MS) 的模型。白细胞介素-27 (IL-27) 抑制 Th17 活性并破坏引起自身免疫的效应 T 细胞的正常活性。蜂毒 (BV) 自古希腊和中国时期以来就被用作一种药物形式。BV 和 BV 衍生的活性成分可能对难治性免疫和神经退行性疾病，如 MS 具有潜在的治疗作用。本研究旨在探讨伊朗蜜蜂毒液对 EAE 小鼠进展的影响。最初，通过用髓鞘少突胶质细胞糖蛋白 35-55 (MOG) 在完全弗氏佐剂 (CFA) 中的乳液免疫 12 只雌性 C57BL/6 小鼠，诱导 EAE，然后用百日咳毒素 (PTx) 在磷酸盐缓冲液中进行。出现临床症状后，通过腹腔内给予 BV 进行治疗。进行了组织病理学和免疫学研究，并在 9-14 天内在动物中诱导 EAE。结果表明，EAE 诱导后小鼠的 IL-27 水平显着降低。然而，与对照组相比，BV 治疗的小鼠的 IL-27 显着增加。组织病理学结果表明，BV 治疗后大脑实质中的炎症细胞数量减少。基于本研究的结果，BV 可能是治疗炎症性疾病如 MS 的合适候选药物。
