Kim Hyoungsoo, Lee Gilgu, Song Junyong, Kim Sang-Gyu
Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
Front Plant Sci. 2022 Apr 18;13:835305. doi: 10.3389/fpls.2022.835305. eCollection 2022.
Flowers emit a bouquet of volatiles to attract pollinators or to protect flowers from pathogen and herbivore attacks. Most floral volatiles are synthesized in the cytoplasm of petals and released into the headspace at a specific time of day. Various floral scent sampling methods coupled with gas chromatography-mass spectrometry have been used to measure the quality and quantity of floral volatiles. However, little is known about the emission patterns of floral scents. In most cases, it is still unclear whether floral scents emit continuously or discontinuously. Here we measured the frequency with which lily flowers emit scents using optical interferometry. By analyzing the refractive index difference between volatile organic compounds and ambient air, we were able to visualize the accumulation of the volatile vapors. The frequency of volatile emission was calculated from the unique footprint of temporal power spectrum maps. Based on these real-time measurements, we found that lily flowers emit the volatile compounds discontinuously, with pulses observed around every 10-50 min.
花朵释放出一束挥发性物质以吸引传粉者，或保护花朵免受病原体和食草动物的侵害。大多数花香挥发性物质在花瓣细胞质中合成，并在一天中的特定时间释放到顶空。各种花香采样方法与气相色谱 - 质谱联用已被用于测量花香挥发性物质的质量和数量。然而，关于花香的释放模式知之甚少。在大多数情况下，花香是持续释放还是间断释放仍不清楚。在这里，我们使用光学干涉测量法测量了百合花释放香味的频率。通过分析挥发性有机化合物与周围空气之间的折射率差异，我们能够可视化挥发性蒸汽的积累。挥发性物质释放频率是根据时间功率谱图的独特特征计算得出的。基于这些实时测量，我们发现百合花间断地释放挥发性化合物，每隔10 - 50分钟左右会观察到脉冲。
