School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, 110032, China.
China Communication Technology (Jiangmen) Co., Ltd., Jiangmen, Guangdong, 529000, China.
J Chromatogr A. 2022 Jun 21;1673:463118. doi: 10.1016/j.chroma.2022.463118. Epub 2022 May 10.
This study focused on developing a set of quality evaluation methods that can reflect the multi-levels and multi-characteristics of traditional Chinese medicine (TCM). Taking licorice (Glycyrrhiza glabra L.) as the method development sample, the feasibility of multi-markers assay by monolinear method (MAML) was explored through the standard curve relationship among active components for the first time. Using glycyrrhizic acid as measurement marker, MAML can simultaneously quantify five components of licorice, including isoliquiritigenin, isoliquiritin apioside, liquiritigenin, liquiritin and liquiritin apioside. Comparing MAML and quantitative analysis of multi-components by single-marker (QAMS) to the external standard method (ESM) respectively, it was found that there was no significant difference in the content of components that were calculated by MAML and ESM (the relative error (RE) was generally less than 2.00%). However, the RE of the component content calculated by QAMS fluctuated greatly, indicating that the MAML was more accurate than QAMS. In addition, UV and THz quantum fingerprints were initiated by the interval erasure method. Taking the systematically quantified fingerprint method as the core, a "Trinity" fingerprint quality evaluation system based on HPLC, UV and THz was developed. The system successfully distinguished the quality differences of licorice samples from 13 producing areas and two ecological models by the comprehensive evaluation results. Simultaneously, the quality information of licorice at different technical levels was revealed. Finally, bivariate correlation analysis was used to examine the linkage between UV/HPLC and antioxidant spectrum efficacy, and the two-dimensional activity spectrum of licorice was provided. It may furnish a more thorough and objective analytical technique for licorice and even other TCMs in chemical fingerprint features, chemical bond vibration characteristics and biological activity information.
本研究聚焦于开发一套能够反映中药多层次、多特征的质量评价方法。以甘草（Glycyrrhiza glabra L.）为方法开发样本，首次通过标准曲线关系探索了单线性法（MAML）进行多标志物分析的可行性。采用甘草酸作为测量标志物，MAML 可以同时定量甘草中的五种成分，包括异甘草素、异甘草苷、甘草素、甘草苷和甘草苷。将 MAML 与多成分定量分析单标志物（QAMS）分别与外标法（ESM）进行比较，发现 MAML 和 ESM 计算的成分含量无显著差异（相对误差（RE）通常小于 2.00%）。然而，QAMS 计算的成分含量的 RE 波动较大，表明 MAML 比 QAMS 更准确。此外，通过间隔擦除法启动了 UV 和太赫兹量子指纹。以系统定量指纹方法为核心，建立了基于 HPLC、UV 和太赫兹的“三位一体”指纹质量评价系统。该系统成功地通过综合评价结果区分了来自 13 个产地和两种生态模式的甘草样品的质量差异。同时，揭示了不同技术水平的甘草质量信息。最后，采用二元相关分析检验了 UV/HPLC 与抗氧化光谱功效之间的联系，提供了甘草的二维活性图谱。它可能为甘草甚至其他中药在化学指纹特征、化学键振动特征和生物活性信息方面提供更全面和客观的分析技术。
