Liu Wei, Zhu Ji-Jun, Liu Yun-Yun, Tang Na-Na, Wang Yan, Jiang Bo, Wang Li-Li, Wang Yu-Xin, Xie Min-Peng, Wang Xiao-Yan
Department of Gastroenterology, Suqian First Hospital, Suqian, China.
J Gastrointest Oncol. 2022 Apr;13(2):559-568. doi: 10.21037/jgo-22-82.
BACKGROUND: Gastric cancer is one of the most lethal cancers. Aberrant expression levels of genes are frequently associated with cell immortalization and the occurrence of tumors. In this study, we aimed to investigate the role of tankyrase 1 () in gastric adenocarcinoma and clarify the underlying mechanism. METHODS: The messenger RNA (mRNA) levels of , human telomerase reverse transcriptase (), and telomeric repeat binding factor 1 () in clinical specimens and SGC-7901 cells were measured via real-time quantitative polymerase chain reaction (RT-qPCR). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunohistochemistry (IHC) assays were utilized to observe the cell apoptosis as well as Ki67 and expression in tumor-bearing models. The effects of antisense oligonucleotides (TANK1 ASODN) on viability and apoptosis of SGC 7901 cells were evaluated by cell counting kit-8 and flow cytometry analysis. RESULTS: We found that and were both increased in gastric adenocarcinoma, while was decreased. Tumor-bearing models demonstrated that TANK1 ASODN appeared to be effective in inhibiting tumor growth and decreasing the expression of . Additionally, TANK1 ASODN inhibited the viability and promoted apoptosis of SGC-7901 cells. Moreover, the mRNA levels of and were modulated by TANK1 ASODN. CONCLUSIONS: This study revealed that TANK1 ASODN inhibits the proliferation and induced the apoptosis of gastric adenocarcinoma cells via manipulating the expression levels of and .
背景：胃癌是最致命的癌症之一。基因表达水平异常常与细胞永生化和肿瘤发生相关。在本研究中，我们旨在探讨端锚聚合酶1（TNKS1）在胃腺癌中的作用并阐明其潜在机制。 方法：通过实时定量聚合酶链反应（RT-qPCR）检测临床标本和SGC-7901细胞中TNKS1、人端粒酶逆转录酶（hTERT）和端粒重复结合因子1（TRF1）的信使核糖核酸（mRNA）水平。利用末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）和免疫组织化学（IHC）分析观察荷瘤模型中的细胞凋亡以及Ki67和hTERT表达。通过细胞计数试剂盒-8和流式细胞术分析评估TNKS1反义寡核苷酸（TANK1 ASODN）对SGC 7901细胞活力和凋亡的影响。 结果：我们发现胃腺癌中TNKS1和hTERT均升高，而TRF1降低。荷瘤模型表明TANK1 ASODN似乎能有效抑制肿瘤生长并降低hTERT表达。此外，TANK1 ASODN抑制SGC-7901细胞的活力并促进其凋亡。而且，TANK1 ASODN调节了hTERT和TRF1的mRNA水平。 结论：本研究表明，TANK1 ASODN通过调控hTERT和TRF1的表达水平抑制胃腺癌细胞的增殖并诱导其凋亡。
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